One-Component Styrene Monooxygenases: An Evolutionary View on a Rare Class of Flavoproteins

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• 作者：Dirk Tischler (1) dirk-tischler@email.de
  Janosch A. D. Gr?ning (1)
  Stefan R. Kaschabek (1)
  Michael Schl?mann (1)
• 关键词：One ; component styrene monooxygenase – Rhodococcus opacus – Self ; sufficient fusion protein – Covalent evolution – Enantioselective epoxidation – Flavin monooxygenase
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2012
• 出版时间：July 2012
• 年：2012
• 卷：167
• 期：5
• 页码：931-944
• 全文大小：589.3 KB
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• 作者单位：1. Interdisciplinary Ecological Center, TU Bergakademie Freiberg, Leipziger Str. 29, 09599 Freiberg, Germany
• ISSN：1559-0291

文摘

Styrene monooxygenases (SMOs) are catalysts for the enantioselective epoxidation of terminal alkenes. Most representatives comprise a reductase and a monooxygenase which are encoded by separate genes (styA, styB). Only six presumed self-sufficient one-component SMOs (styA2B) have previously been submitted to databases, and one has so far been characterized. StyA2B can be supported by another epoxidase (StyA1) encoded by styA1, a gene in direct neighborhood of styA2B. The present report describes the identification of a further styA1/styA2B-like SMO, which was detected in Rhodococcus opacus MR11. Based on the initially available sequences of styA2B-type SMOs, primers directed at conserved sequences were designed and a 7,012-bp genomic fragment from strain MR11 was obtained after PCRs and subsequent genome walking. Six open reading frames (ORFs) were detected and compared to genomic fragments of strains comprising either two- or one-component SMOs. Among the proteins encoded by the ORFs, the monooxygenase StyA1/StyA2B showed the highest divergence on amino acid level when comparing proteins from different sources. That finding, a rare distribution of styA2B genes among bacteria, and the general observation of evolution from simple to complex systems indicate that one-component SMOs evolved from two-component ancestors. Analysis of gene products from styA/styB- and styA1/styA2B-like SMOs revealed that a fusion of styA/styB to styA2B might have happened at least twice among microorganisms. This points to a convergent evolution of one-component SMOs.