Development of an in vivo tissue-engineered valved conduit (type S biovalve) using a slitted mold

详细信息    查看全文

• 作者：Marina Funayama ; Maya Furukoshi ; Takeshi Moriwaki…
• 关键词：Biovalve ; Connective tissue ; In ; body tissue architecture ; Leaflet ; Sinus of Valsalva
• 刊名：Journal of Artificial Organs
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：18
• 期：4
• 页码：382-386
• 全文大小：7,268 KB
• 参考文献：1.Hayashida K, Kanda K, Oie T, Okamoto Y, Ishibashi-Ueda H, Onoyama M, Tajikawa T, Ohba K, Yaku H, Nakayama Y. Architecture of an in vivo-tissue engineered autologous conduit “biovalve- J Biomed Mater Res B Appl Biomater. 2008;86:1-.CrossRef PubMed
  2.Nakayama Y, Yamanami M, Yahata Y, Tajikawa T, Ohba K, Watanabe T, Kanda K, Yaku H. Preparation of a completely autologous trileaflet valve-shaped construct by in-body tissue architecture technology. J Biomed Mater Res B Appl Biomater. 2009;91:813-.CrossRef PubMed
  3.Takewa Y, Yamanami M, Kishimoto Y, Arakawa M, Kanda K, Matsui Y, Oie T, Ishibashi-Ueda H, Tajikawa T, Ohba K, Yaku H, Taenaka Y, Tatsumi E, Nakayama Y. In vivo evaluation of an in-body, tissue-engineered, completely autologous valved conduit (biovalve type VI) as an aortic valve in a goat model. J Artif Organs. 2013;16:176-4.CrossRef PubMed
  4.Yamanami M, Yahata Y, Uechi M, Fujiwara M, Ishibashi-Ueda H, Kanda K, Watanabe T, Tajikawa T, Ohba K, Yaku H, Nakayama Y. Development of a completely autologous valved conduit with the sinus of Valsalva using in-body tissue architecture technology: a pilot study in pulmonary valve replacement in a beagle model. Circulation. 2010;122:S100-.CrossRef PubMed
  5.Nakayama Y, Takewa Y, Sumikura H, Yamanami M, Matsui Y, Oie T, Kishimoto Y, Arakawa M, Ohmuma K, Tajikawa T, Kanda K, Tatsumi E. In-body tissue-engineered aortic valve (Biovalve type VII) architecture based on 3D printer molding. J Biomed Mater Res B Appl Biomater. 2015;103:1-1.CrossRef PubMed
  6.Mizuno T, Takewa Y, Sumikura H, Ohnuma K, Moriwaki T, Yamanami M, Oie T, Tatsumi E, Uechi M, Nakayama Y. Preparation of an autologous heart valve with a stent (stent-biovalve) using the stent eversion method. J Biomed Mater Res B Appl Biomater. 2014;102:1038-5.CrossRef PubMed
  7.Funayama M, Sumikura H, Takewa Y, Tatsumi E, Nakayama Y. Development of self-expanding valved stents with autologous tubular leaflet tissues for transcatheter valve implantation. J Artif Organs. 2015;. doi:10.-007/?s1004701508206 .
  8.Stickler P, De Visscher G, Mesure L, Famaey N, Martin D, Campbell JH, Van Oosterwyck H, Meuris B, Flameng W. Cyclically stretching developing tissue in vivo enhances mechanical strength and organization of vascular grafts. Acta Biomater. 2010;6:2448-6.CrossRef PubMed
  9.Kishi A, Isoyama T, Saito I, Miura H, Nakagawa H, Kouno A, Ono T, Inoue Y, Yamaguchi S, Shi W, Abe Y, Imachi K, Noshiro M. Use of in vivo insert molding to form a jellyfish valve leaflet. Artif Organs. 2010;34:1125-1.CrossRef PubMed
  10.Funayama M, Takewa Y, Oie T, Matsui Y, Tatsumi E, Nakayama Y. In situ observation and enhancement of leaflet tissue formation in bioprosthetic “biovalve- J Artif Organs. 2015;18:40-.CrossRef PubMed
  11.Grainger DW. All charged up about implanted biomaterials. Nat Biotechnol. 2013;31:507-.CrossRef PubMed
  12.Sripathi VC, Kumar RK, Balakrishnan KR. Further insights into normal aortic valve function: role of a compliant aortic root on leaflet opening and valve orifice area. Ann Thorac Surg. 2004;77:844-1.CrossRef PubMed
  
• 作者单位：Marina Funayama (1)
  Maya Furukoshi (1)
  Takeshi Moriwaki (1)
  Yasuhide Nakayama (1)
  
  1. Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka, 565-8565, Japan
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Surgery
  Cardiac Surgery
  Biomedical Engineering
  Orthopedics
  Nephrology
  Hepatology
  
• 出版者：Springer Japan
• ISSN：1619-0904

文摘

In autologous valved conduits (biovalves) using in-body tissue architecture, the limited area available for leaflet formation is a concern. In this study, we designed a novel biovalve mold with slits to enhance in vivo cell migration, regardless of size. As a control, the original mold without slits was used. When both types of molds were embedded into subcutaneous pouches in beagle dogs for 8 weeks, the outer surfaces of all molds were completely covered with connective tissue to form conduit tissue. In the molds without slits, the leaflet size was limited to half of the design. In contrast, in the mold with slits, the complete leaflet area was formed. Upon trimming excess peripheral tissues, removing the mold, and cutting the connective tissue formed at the slits, completely autologous connective tissue biovalves with the designed leaflet area were obtained as type S (diameter, 6-8 mm) biovalves. The slit structure customized to the mold was effective for allowing cells to enter, thereby facilitating cell migration and contributing to the successful preparation of reliable biovalves of various physiological sizes suitable for all clinical uses. Keywords Biovalve Connective tissue In-body tissue architecture Leaflet Sinus of Valsalva