Development of an in vivo tissue-engineered vascular graft with designed wall thickness (biotube type C) based on a novel caged mold

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• 作者：Maya Furukoshi ; Takeshi Moriwaki ; Yasuhide Nakayama
• 关键词：Small ; diameter ; Vascular grafts ; Biotube ; In vivo tissue engineering
• 刊名：Journal of Artificial Organs
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：19
• 期：1
• 页码：54-61
• 全文大小：1,453 KB
• 参考文献：1.Esquivel CO, Blaisdell FW. Why small vascular grafts fail: a review of clinical and experimental experience and the significance of the interaction of blood at the interface. J Surg Res. 1986;41:1–15.CrossRef PubMed
  2.Watanabe T, Kanda K, Yamanami M, Ishibashi-Ueda H, Yaku H, Nakayama Y. Long-term animal implantation study of biotube-autologous small-caliber vascular graft fabricated by in-body tissue architecture. J Biomed Mater Res B Appl Biomater. 2011;98:120–6.CrossRef PubMed
  3.Yamanami M, Ishibashi-Ueda H, Yamamoto A, Iida H, Watanabe T, Kanda K, Yaku H, Nakyama Y. Implantation study of small-caliber “biotube” vascular grafts in a rat model. J Artif Organs. 2013;16:59–65.CrossRef PubMed
  4.Sakai O, Kanda K, Takamizawa K, Sato T, Yaku H, Nakayama Y. Faster and stronger vascular “Biotube” graft fabrication in vivo using a novel nicotine-containing mold. J Biomed Mater Res B Appl Biomater. 2009;90:412–20.PubMed
  5.Nakayama Y, Tsujinaka T. Acceleration of robust “biotube” vascular graft fabrication by in-body tissue architecture technology using novel eosin Y-releasing mold. J Biomed Mater Res B Appl Biomater. 2014;102:231–8.CrossRef PubMed
  6.Sakai O, Kanda K, Ishibashi-Ueda H, Takamizawa K, Ametani A, Yaku H, Nakayama Y. Development of the wing-attached rod for acceleration of “Biotube” vascular grafts fabrication in vivo. J Biomed Mater Res B Appl Biomater. 2007;83:240–7.CrossRef PubMed
  7.Oie T, Yamanami M, Ishibahi-Ueda H, Kanda K, Yaku H, Nakayama Y. In-body optical stimulation formed connective tissue vascular grafts, “biotubes”, with many capillaries and elastic fibers. J Artif Organs. 2010;13:235–40.CrossRef PubMed
  8.Hayashi K, Handa H, Nagasawa S, Okumura A, Moritake K. Stiffness and elastic behavior of human intracranial and extracranial arteries. J Biomech. 1980;13:175–84.CrossRef PubMed
  9.Hayashi K, Nakamura T. Material test system for the evaluation of mechanical properties of biomaterials. J Biomed Mater Res. 1985;19:133–44.CrossRef PubMed
  10.Hayashi K, Igarashi Y, Takamizawa K. Mechanical properties and hemodynamics in coronary arteries. In: Kitamura K, Abe H, Sagawa K, editors. New approaches in cardiac mechanics. Tokyo: Japan Scientific Societies Press; 1986. p. 285–94.
  11.Nakayama Y, Takewa Y, Sumikura H, Yamnami M, Matui Y, Oie T, Kishimoto Y, Arakawa M, Ohmura K, Kajikawa T, Kanda K, Tatsumi E. In-body tissue-engineered aortic valve (Biovalve type VII) architecture based on 3D printer molding. J Biomed Mater Res B Appl Biomater. 2015;103:1–11.CrossRef PubMed
  12.Funayama M, Takewa Y, Oie T, Matsui Y, Tatsumi E, Nakayama Y. In situ observation and enhancement of leaflet tissue formation in bioprosthetic “biovalve”. J Artif Organs. 2015;18:40–7.CrossRef PubMed
  13.Madras PN, Ward CA, Johnson WR, Singh PI. Anastomotic hyperplasia. Surgery. 1981;90:922–3.PubMed
  14.Watanabe T, Kanda K, Ishibashi-Ueda H, Yaku H, Nakayama Y. Autologous small-caliber “biotube” vascular grafts with argatroban loading: a histomorphological examination after implantation to rabbits. J Biomed Mater Res B Appl Biomater. 2010;92:236–42.CrossRef PubMed
  15.Klinkert P, van Dijk PJ, Breslau PJ. Polytetrafluoroethylene femorotibial bypass grafting: 5-year patency and limb salvage. Ann Vasc Surg. 2003;17:486–91.CrossRef PubMed
  16.Arena FJ. Arterial kink and damage in normal segments of the superficial femoral and popliteal arteries abutting nitinol stents-a common cause of late occlusion and restenosis? A single-center experience. J Invasive Cardiol. 2005;17:482–6.PubMed
  17.Campbell GR, Campbell JH. Development of tissue engineered vascular grafts. Curr Pharm Biotechnol. 2007;8:43–50.CrossRef PubMed
  
• 作者单位：Maya Furukoshi (1)
  Takeshi Moriwaki (1)
  Yasuhide Nakayama (1)
  
  1. Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka, 565-8565, Japan
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Surgery
  Cardiac Surgery
  Biomedical Engineering
  Orthopedics
  Nephrology
  Hepatology
  
• 出版者：Springer Japan
• ISSN：1619-0904

文摘

Small-diameter biotube vascular grafts developed by in-body tissue architecture had high patency at implantation into rabbit carotid arteries or rat abdominal aortas. However, the thin walls (34 ± 14 μm) of the original biotubes made their implantation difficult into areas with low blood flow volumes or low blood pressure due to insufficient mechanical strength to maintain luminal shape. In this study, caged molds with several windows were designed to prepare more robust biotubes. The molds were assembled with silicone tubes (external diameter 2 mm) and cylindrical covers (outer diameter 7 mm) with 12 linear windows (1 × 9 mm). After the molds were embedded into beagle dorsal subcutaneous pouches for 4 weeks, type C (cage) biotubes were obtained by completely extracting the surrounding connective tissues from the molds and removing the molds. The biotube walls (778 ± 31 μm) were formed at the aperture (width 1 mm) between the silicone rods and the covers by connective cell migration through the windows of the covers. Excellent mechanical properties (external pressure resistance, approximately 4 times higher than beagle native femoral arteries; burst strength, approximately 2 times higher than original biotubes) were obtained. In the acute phase of implantation of the biotubes into beagle femoral arteries, perfect patency was obtained with little stenosis and no aneurysmal dilation. The type C biotubes may be useful for implantation into peripheral arteries or veins in addition to aortas.