Influence of different trap solutions on the determination of root exudates in Lupinus albus L.

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• 作者：Fabio Valentinuzzi ; Stefano Cesco ; Nicola Tomasi…
• 关键词：Root exudates ; Trap solutions ; Lupinus albus L. ; Organic acids ; Flavonoids
• 刊名：Biology and Fertility of Soils
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：51
• 期：6
• 页码：757-765
• 全文大小：226 KB
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• 作者单位：Fabio Valentinuzzi (1)
  Stefano Cesco (1)
  Nicola Tomasi (2)
  Tanja Mimmo (1)
  
  1. Faculty of Science and Technology, Free University of Bozen-Bolzano, Piazza Università 5, 39100, Bolzano, Italy
  2. Dipartimento di Scienze Agrarie e Ambientali, University of Udine, Via delle Scienze 208, 33100, Udine, Italy
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Life Sciences
  Agriculture
  Soil Science and Conservation
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0789

文摘

White lupin is very often used as a model plant for root exudation studies due to its capability to release huge amounts of organic acids and flavonoids. The complex nature of these organic compounds makes not only their analytical determination difficult but also their extraction from soil samples. For these reasons simplified approaches, as hydroponic-based systems are widely used to study the root exudation. Therefore, the composition of a trap solution is crucial to limit artefacts causing over/underestimation of exudation rates and/or a biased molecular composition of the collected compounds. The present study was aimed at assessing the influence of different trap solutions and collection times on the quali- and quantitative root exudation pattern of white lupin (Lupinus albus L.) grown under phosphorus (P) and iron (Fe) deficiency. Our results suggest that, in works aimed at studying root exudation processes, water is the most effective trap solution to collect the exudates like organic acids and flavonoids, especially in short time (e.g. 2?h). For longer times, low concentrations of Ca could be helpful to limit osmotic stress and possible passive leakage and/or diffusion. The use of bacteriostatic compounds as NaN3 and Micropur bias the results, due to interferences either with the metabolism or inhibition of the exudation processes, especially in the case of flavonoids such as quercetin. Also, the use of a pH buffer solution like 2-(N-morpholino)ethanesulfonic acid (MES) should be avoided for its undesired interferences with the release.