Human IgG adsorption using dye-ligand epoxy chitosan/alginate as adsorbent: influence of buffer system

详细信息    查看全文

• 作者：Diego R. Gondim (1)
  Natália A. Dias (1)
  Igor T. L. Bresolin (2)
  Andreza M. Baptistiolli (2)
  Diana C. S. Azevedo (1)
  Ivanildo J. Silva Jr. (1)
  
• 关键词：Adsorption ; Dye ; ligand affinity chromatography ; Human immunoglobulin G ; Human serum ; Cibacron blue ; Chitosan
• 刊名：Adsorption
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：20
• 期：8
• 页码：925-934
• 全文大小：791 KB
• 参考文献：1. Adriano, W.S., Mendon?a, D.B., Rodrigues, D.S., Mammarella, E.J., Giordano, R.L.C.: Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin. Biomacromolecules 9, 2170-179 (2008) 21/bm8002754" target="_blank" title="It opens in new window">CrossRef
  2. Subramanian, A., Rau, A.V., Kaligotla, H.: Surface modification of chitosan for selective surface-protein interaction. Carbohy. Polym. 66, 321-32 (2006) 2006.03.022" target="_blank" title="It opens in new window">CrossRef
  3. Chen, A.-H., Liu, S.-C., Chen, C.-Y., Chen, C.-Y.: Comparative adsorption of Cu(II), Zn(II) and Pb(II) ions in aqueous solution on the crosslinked chitosan with epichlohydrin. J. Hazar. Mat. 154, 184-91 (2008) 2007.10.009" target="_blank" title="It opens in new window">CrossRef
  4. Bayramo?lu, G., Yal?in, E., Arica, M.Y.: Adsorption of serum albumin and γ-globulin from single and binary mixture and characterization of pHEMA-based affinity membrane surface by contact angle measurements. Biochem. Eng. J. 26, 12-1 (2005) 2005.06.002" target="_blank" title="It opens in new window">CrossRef
  5. Bayramo?lu, G., Oktem, H.A., Arica, M.Y.: A dye–ligand immobilized poly(2-hydroxyethylmethacrylate) membrane used for adsorption and isolation of immunoglobulin G. Biochem. Eng. J. 34, 147-55 (2007) 2006.11.025" target="_blank" title="It opens in new window">CrossRef
  6. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-54 (1976) 2697(76)90527-3" target="_blank" title="It opens in new window">CrossRef
  7. Burnouf, T., Radosevich, M.: Affinity chromatography in the industrial purification of plasma proteins for therapeutic use. J. Biochem. Biophys. Methods 49, 575-86 (2001) 22X(01)00221-4" target="_blank" title="It opens in new window">CrossRef
  8. Cetinus, S.A., ?ztop, H.N., Saraydin, D.: Immobilization of catalase onto chitosan and cibacron blue F3GA attached chitosan beads. Enzyme. Micro. Technol. 41, 447-54 (2007) 2007.03.014" target="_blank" title="It opens in new window">CrossRef
  9. Chaves, J.A.P., Bezerra, C.W.B., Silva, H.A.S., Santana, S.A.A.: Caracteriza??o e aplica??o do biopolímero quitosana como removedor de corante têxtil presente em meio aquoso. Cad. Pesq. 16(2), 36-3 (2009)
  10. Denizli, A., Piskin, E.: Dye-ligand affinity systems. J. Biolchem. Bioph. Methods 49, 391-16 (2001) 22X(01)00209-3" target="_blank" title="It opens in new window">CrossRef
  11. Feng, Z., Shao, Z., Yao, J., Huang, Y., Chen, X.: Protein adsorption and separation with chitosan-based amphoteric membranes. Polymer 50, 1257-263 (2009) 2008.12.046" target="_blank" title="It opens in new window">CrossRef
  12. Ghose, S., Tao, Y.Y., Conley, L., Cecchini, D.: Purification of monoclonal antibodies by hydrophobic interaction chromatography under no-salt conditions. mAbs 5(5), 795-00 (2013) 25552" target="_blank" title="It opens in new window">CrossRef
  13. Gondim, D.R., Lima, L.P., Souza, M.C.M., Bresolin, I.T.L., Adriano, W.S., Azevedo, D.C.S., Silva, I.J.: Dye ligand epoxide chitosan/alginate: a potential new stationary phase for human IgG purification. Adsorp. Sci. Technol. 30, 701-12 (2012) 260/0263-6174.30.8-9.701" target="_blank" title="It opens in new window">CrossRef
  14. Hoven, V.P., Tangpasuthadol, V., Angkitpaiboon, Y., Vallapa, N., Kiatkamjornwong, S.: Surface-charged chitosan: preparation and protein adsorption. Carbohydr. Polym. 68, 44-3 (2007) 2006.07.008" target="_blank" title="It opens in new window">CrossRef
  15. Ka
• 作者单位：Diego R. Gondim (1)
  Natália A. Dias (1)
  Igor T. L. Bresolin (2)
  Andreza M. Baptistiolli (2)
  Diana C. S. Azevedo (1)
  Ivanildo J. Silva Jr. (1)
  
  1. Grupo de Pesquisa em Separa??es por Adsor??o (GPSA), Departamento de Engenharia Química, Centro de Tecnologia, Universidade Federal do Ceará, Campus do Pici, Bl. 709, Fortaleza, CE, 60455-760, Brazil
  2. Departamento de Ciências Exatas e da Terra, Universidade Federal de S?o Paulo, Campus de Diadema, Diadema, SP, 09972-270, Brazil
  
• ISSN：1572-8757

文摘

The aim of this work was to evaluate the ability of Cibacron Blue F-3GA dye-ligand chitosan/alginate epoxide on human IgG adsorption under different buffer systems at 25?mmol?L?: Morpholinoethane sulfonic acid, morpholinopropane sulfonic acid, hydroxyethylpiperazine ethanesulfonic acid and tris-hydroxymethyl aminomethane (Tris-a href='/search?dc.title=HCl&facet-content-type=ReferenceWorkEntry&sortOrder=relevance' class='reference-link webtrekk-track' gaCategory="Internal link" gaLabel="HCl" gaAction="reference keyword">HCl). Batch adsorption studies were performed in order to evaluate the effect of buffer pH and nature on IgG uptake as well the equilibrium isotherms. Chromatographic experiments were performed with both human IgG (high purity) and human serum and each buffer with NaCl 1.0?M was used in the elution step. Best results (maximum equilibrium adsorption capacity of 110.9?mg?g?) were found at pH 7.8 with Tris-a href='/search?dc.title=HCl&facet-content-type=ReferenceWorkEntry&sortOrder=relevance' class='reference-link webtrekk-track' gaCategory="Internal link" gaLabel="HCl" gaAction="reference keyword">HCl buffer. The Langmuir–Freundlich model provided a good fit for the adsorption isotherm. In chromatographic experiments with high purity IgG and Tris/HCl buffer, IgG dynamic adsorption capacity onto E-Ch/Al-Cibacron was 13.4?mg?g?, which accounted for 60?% of IgG injected into the column. Chromatographic experiments with diluted (tenfold) human serum were conducted and it was found by electrophoresis that IgG is preferentially adsorbed with respect to other proteins using all buffers systems, especially with Tris-a href='/search?dc.title=HCl&facet-content-type=ReferenceWorkEntry&sortOrder=relevance' class='reference-link webtrekk-track' gaCategory="Internal link" gaLabel="HCl" gaAction="reference keyword">HCl. The amount of desorbed protein from E-Ch/Al-Cibacron was around 7.0?mg?g? for all buffers. The new stationary phase showed high affinity for IgG and may be a potential adsorbent for human IgG purification.