No evidence for effects of formalin storage duration or solvent medium exposure on avian sperm morphology

详细信息    查看全文

• 作者：Tim Schmoll ; Romina Sanciprian ; Oddmund Kleven
• 关键词：DMEM ; Formalin preservation ; Museum collections ; PBS ; Sperm morphology ; Sperm storage duration
• 刊名：Journal of Ornithology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：157
• 期：2
• 页码：647-652
• 全文大小：723 KB
• 参考文献：Albrecht T, Kleven O, Kreisinger J, Laskemoen T, Omotoriogun TC, Ottosson U, Reif J, Sedláček O, Hořák D, Robertson RJ, Lifjeld JT (2013) Sperm competition in tropical versus temperate zone birds. Proc R Soc Lond B 280:20122434. doi:10.​1098/​rspb.​2012.​2434 CrossRef
  Bates D, Maechler M, Bolker B, Walker S (2014) lme4: Linear mixed-effects models using Eigen and S4. R package version 1.1-7. http://​CRAN.​R-project.​org/​package=​lme4
  Birkhead TR, Hosken DJ, Pitnick S (2009) Sperm biology—an evolutionary perspective. Academic Press, Oxford
  Briskie JV, Birkhead TR (1993) A review of methods used to study the anatomy of avian sperm storage. Ornis Scan 24:323–329. doi:10.​2307/​3676795 CrossRef
  Cramer ERA, Laskemoen T, Kleven O, Lifjeld JT (2013) Sperm length variation in House Wrens Troglodytes aedon. J Ornithol 154:129–138. doi:10.​1007/​s10336-012-0878-3 CrossRef
  Helfenstein F, Losdat S, Møller AP, Blount JD, Richner H (2010a) Sperm of colourful males are better protected against oxidative stress. Ecol Lett 13:213–222. doi:10.​1111/​j.​1461-0248.​2009.​01419.​x CrossRef PubMed
  Helfenstein F, Podevin M, Richner H (2010b) Sperm morphology, swimming velocity, and longevity in the House sparrow Passer domesticus. Behav Ecol Sociobiol 64:557–565. doi:10.​1007/​s00265-009-0871-x CrossRef
  Hermosell IG, Laskemoen T, Rowe M, Møller AP, Mousseau TA, Albrecht T, Lifjeld JT (2013) Patterns of sperm damage in Chernobyl passerine birds suggest a trade-off between sperm length and integrity. Biol Lett 9(2013):0530. doi:10.​1098/​rsbl.​2013.​0530
  Immler S, Pryke SR, Birkhead TR, Griffith SC (2010) Pronounced within-individual plasticity in sperm morphometry across social environments. Evolution 64:1634–1643. doi:10.​1111/​j.​1558-5646.​2009.​00924.​x CrossRef PubMed
  Immler S, Gonzalez-Voyer A, Birkhead TR (2012) Distinct evolutionary patterns of morphometric sperm traits in passerine birds. Proc R Soc Lond B 279:4174–4182. doi:10.​1098/​rspb.​2012.​1398 CrossRef
  Jonmarker S, Valdman A, Lindberg A, Hellström M, Egevad L (2006) Tissue shrinkage after fixation with formalin injection of prostatectomy specimens. Virchows Arch 449:297–301. doi:10.​1007/​s00428-006-0259-5 CrossRef PubMed
  Laskemoen T, Kleven O, Fossøy F, Lifjeld JT (2007) Intraspecific variation in sperm length in two passerine species, the Bluethroat Luscinia svecica and the Willow Warbler Phylloscopus trochilus. Ornis Fenn 84:131–139
  Laskemoen T, Albrecht T, Bonisoli-Alquati A, Cepak J, de Lope F, Hermosell IG, Johannessen LE, Kleven O, Marzal A, Mousseau TA, Møller AP, Robertson RJ, Rudolfsen G, Saino N, Vortman Y, Lifjeld JT (2013a) Variation in sperm morphometry and sperm competition among Barn swallow (Hirundo rustica) populations. Behav Ecol Sociobiol 67:301–309. doi:10.​1007/​s00265-012-1450-0 CrossRef
  Laskemoen T, Kleven O, Johannessen LE, Fossøy F, Robertson RJ, Lifjeld JT (2013b) Repeatability of sperm size and motility within and between seasons in the Barn Swallow (Hirundo rustica). J Ornithol 154:955–963. doi:10.​1007/​s10336-013-0961-4 CrossRef
  Lessels CM, Boag PT (1987) Unrepeatable repeatabilities: a common mistake. Auk 104:116–121CrossRef
  Lifjeld JT, Laskemoen T, Kleven O, Pedersen ATM, Lampe HM, Rudolfsen G, Schmoll T, Slagsvold T (2012) No evidence for pre-copulatory sexual selection on sperm length in a passerine bird. PLoS ONE 7:e32611. doi:10.​1371/​journal.​pone.​0032611 CrossRef PubMed PubMedCentral
  Lifjeld JT, Hoenen A, Johannessen LE, Laskemoen T, Lopes RJ, Rodrigues P, Rowe M (2013) The Azores bullfinch (Pyrrhula murina) has the same unusual and size-variable sperm morphology as the Eurasian bullfinch (Pyrrhula pyrrhula). Biol J Linn Soc 108:677–687. doi:10.​1111/​j.​1095-8312.​2012.​02040.​x CrossRef
  Lüpold S, Birkhead TR, Westneat DF (2012) Seasonal variation in ejaculate traits of male red-winged blackbirds (Agelaius phoeniceus). Behav Ecol Sociobiol 66:1607–1617. doi:10.​1007/​s00265-012-1415-3 CrossRef
  Rasband WS (1997–2012) ImageJ. U. S. National Institutes of Health, Bethesda, Maryland, USA. http://​imagej.​nih.​gov/​ij/​
  R Development Core Team (2014) R: a language and environment for statistical computing, v 3.1.1. R Foundation for Statistical Computing, Vienna, Austria. http://​www.​R-project.​org/​
  Schmoll T, Kleven O (2011) Sperm dimensions differ between two coal tit Periparus ater populations. J Ornithol 152:515–520. doi:10.​1007/​s10336-010-0603-z CrossRef
  Wolfson A (1952) The cloacal protuberance—a means for determining breeding condition in live male passerines. Bird Band 23:159–165CrossRef
  
• 作者单位：Tim Schmoll (1)
  Romina Sanciprian (1)
  Oddmund Kleven (2)
  
  1. Evolutionary Biology, Bielefeld University, Morgenbreede 45, 33615, Bielefeld, Germany
  2. Norwegian Institute for Nature Research (NINA), P.O. Box 5685, Sluppen, 7485, Trondheim, Norway
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Zoology
  Animal Ecology
  Evolutionary Biology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：2193-7206

文摘

Morphometric analysis of avian spermatozoa from sperm samples preserved in formalin is a frequently adopted procedure in basic science (e.g. evolutionary ecology) and applied disciplines (e.g. animal breeding). Many research questions such as individual-based longitudinal studies of sperm traits require comparisons of formalin-stored sperm samples collected across multiple sampling events, which may be separated by years. Such analyses presuppose that prolonged storage in formalin does not affect sperm morphology, an assumption often implicitly made in the analysis of avian sperm morphology. This assumption, however, has never been tested, although for many study designs a potential effect of sperm storage duration may well confound the focal analysis. Based on pairwise comparisons of 22 experimental ejaculates from three passerine bird species, we found no evidence that differential storage duration of more than 1 year had affected the total length of spermatozoa stored in a 5 % formaldehyde solution. This suggests that formalin-stored sperm samples from long-term studies or museum collections can be merged in combined analyses without confounding differential storage duration with natural between-year variation in sperm dimensions or age effects in longitudinal studies. Based on pairwise comparisons of 29 split ejaculates, we also found no evidence that spermatozoa differed in length when solved initially in either phosphate buffered saline or Dulbecco’s Modified Eagle Medium, a standard medium for videotaping live sperm, prior to preservation and storage in formalin. Sperm samples treated differently in this respect may thus be merged into combined analyses, too.