Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis)

详细信息    查看全文

• 作者：Hayder Hamzah Ibrahim ; Yusuf Abba ; Ihsan Muneer Ahmed…
• 关键词：Pasteurella multocida B ; 2 ; Female reproductive system ; Mammary gland ; Buffaloes ; Histopathology ; PCR
• 刊名：Comparative Clinical Pathology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：25
• 期：2
• 页码：319-326
• 全文大小：1,884 KB
• 参考文献：Abubakar MS, Zamri-Saad M (2011) Clinico-pathological changes in buffalo calves following oral exposure to Pasteurella multocida B:2. Basic Appl Pathol 4(4):130–135. doi:10.​1111/​j.​1755-9294.​2011.​01113.​x CrossRef
  Abubakar M, Zamri-Saad M, Jasni S (2013) Ultrastructural changes and bacterial localization in buffalo calves following oral exposure to Pasteurella multocida B: 2. Pak Vet J 33(1):101–106
  Annas, S Zamri-Saad, M Jesse, F F and Zunita, Z 2014 New sites of localisation of Pasteurella multocida B: 2 in buffalo surviving experimental haemorrhagic septicaemia. BMC Vet. Res., 10(1), 88.
  Azawi OI (2013) Pathogenesis of postpartum metritis in buffaloes: a review. Buffalo Bull 32(1)
  Azawi O, IOmran SN, IOmran SN, Hadad JJ (2007) Clinical, bacteriological, and histopathological study of toxic puerperal metritis in Iraqi buffalo. J Dairy Sci 90(10):4654–4660. doi:10.​3168/​jds.​2007-0114 CrossRef PubMed
  Benkirane A, De Alwis M (2002) Haemorrhagic septicaemia, its significance, prevention and control in Asia. Vet Med Czech 47(8):234–240
  Boyce JD, Seemann T, Adler B, Harper M (2012) Pathogenomics of Pasteurella multocida. In: Aktories K, Orth JHC, Adler B, Adler B (eds) Pasteurella multocida, vol 361. Springer, Berlin, pp pp. 23–pp. 38CrossRef
  De Alwis MCL (1984) Haemorrhagic septicaemia in cattle and buffaloes. Rev Sci Tech Off  Int Epiz 3:707–730
  De Alwis M (1992a) Pasteurellosis in production animals: a review. Pasteurellosis in production animals. ACIAR Publishing, Canberra, pp 11–22
  De Alwis MCL (1992b) Haemorrhagic septicaemia-a general review. Br Vet J 148(2):99–112. doi:10.​1016/​0007-1935(92)90101-6 CrossRef PubMed
  De Alwis MC (1999) Haemorrhagic septicaemia. Canberra, Australia: Australian Centre for International Agricultural Research (ACIAR).
  Dowling A, Hodgson JC, Schock A, Donachie W, Eckersall PD, Mckendrick IJ (2002) Experimental induction of pneumonic pasteurellosis in calves by intratracheal infection with Pasteurella multocida biotype A:3. Res Vet Sci 73(1):37–44. doi:10.​1016/​S0034-5288(02)00037-1 CrossRef PubMed
  Goldman, E and Green, H (2008) Practical handbook of microbiology (2 ed.): CRC Press
  Harper M, Boyce JD, Adler B (2006) Pasteurella multocida pathogenesis: 125 years after Pasteur. FEMS Microbiol Lett 265(1):1–10CrossRef PubMed
  Jesse FF, Affandi SA, Osman AY, Adamu L, Saad MZ, Haron AW, Omar AR, Sabri J et al (2013a) Clinico-pathological features in mice following oral exposure to Pasteurella multocida B: 2. IOSR J Agric Vet Sci 3(4):35–39CrossRef
  Jesse FFA, Abdinasir Lawan A, Zakaria YO, Abdullah ZR, Zamri MS, Saharee AA (2013b) Clinico-pathological responses of calves associated with infection of Pasteurella multocida type B and the bacterial lipopolysaccharide and outer membrane protein immunogens. Int J Anim Vet Adv 5(5):190–198
  Jesse FFA, Tijjani L, Adamu A, Mohammed K, Abba Y, Sadiq MA, Zainal R, Zainal MJ et al (2014) Hormonal and histopathological alterations in pituitary glands and reproductive organs of male and female mice orally inoculated with Pasteurella multocida type B: 2 and its lipopolysaccharides. Am J Anim Vet Sci 9:200–212
  Jesse FFA, Abba Y, Tijjani A, Sadiq MA, Konto M, Adamu L, Wahid AH, MohdAzmi ML, Eric LTC, Rahman MFA, Mydin NB, Saharee AA (2015) Gonado-hypophyseal lesions and reproductive hormonal changes in Brucella melitensis-infected mice and its lipopolysaccharides (LPSs). Comp Clin Pathol 1–6
  Khaleel MM, Abdullah FFJ, Adamu L, Abba Y, Haron AW, Saad MZ, Omar AR (2014) Histopathological changes in mice infected with river water contaminated by Pasteurella multocida type B:2. Am J Anim Vet Sci 9(2):71CrossRef
  Khan A, Saleemi MK, Khan MZ, Gul S, TIrfan M, Qamar MS (2011) Haemorrhagic septicemia in buffalo (Bubalus bubalis) calves under sub-tropical conditions in Pakistan. Pak J Zool 43:295–302
  Khuder Z, Osman AY, Jesse FF, Haron AW, Saharee AA, Sabri J, Yusoff R, and Abdullah R (2012) Sex hormone profiles and cellular changes of reproductive organs of mice experimentally infected with C. pseudotuberculosis and its exotoxin phospholipase D (PLD). IOSR J Agr Vet Sci 1(3):24–29
  Kumar V, Abbas AK, Aster JC (2012) Robbins basic pathology. Elsevier Health Sciences
  Lane EP, Kock ND, Hill FWG, Mohan K (1992) An outbreak of haemorrhagic septicaemia (septicaemic pasteurellosis) in cattle in Zimbabwe. Trop Anim Health Prod 24(2):97–102. doi:10.​1007/​BF02356952 CrossRef PubMed
  Odugbo M, Turaki U, Itodo A, Okwori A, Yakubu R (2005) Experimental haemorrhagic septicemia of calves with Pasteurella multocida Serotype E: 2: clinical, pathologic and microbiologic studies. Rev Elev Med Vet Pays Trop 58(3)
  Othman S, Parton R, Coote J (2012) Interaction between mammalian cells and Pasteurella multocida B:2. Adherence, invasion and intracellular survival. Microb Pathog 52(6):353–358. doi:10.​1016/​j.​micpath.​2012.​03.​005 CrossRef PubMed
  Pampori Z, Pandita S (2013) Reproductive cycle stage bias in physiological and immune responses to endotoxin challenge in Murrah buffaloes (Bubalus bubalis). Buffalo Bull 32(4):270–282
  Perera B (2011) Reproductive cycles of buffalo. Anim Reprod Sci 124(3):194–199CrossRef PubMed
  Ranjan R, Panda S, Acharya A, Singh A, Gupta M (2011) Molecular diagnosis of Haemorrhagic Septicaemia-A Review. Vet World 4(4):189–192
  Townsend KM, Frost AJ, Lee C, WPapadimitriou JM, Dawkins HJ (1998) Development of PCR assays for species-and type-specific identification of Pasteurella multocida isolates. J Clin Microbiol 36(4):1096–1100PubMed PubMedCentral
  Townsend KM, Boyce J, DChung JY, Frost AJ, Adler B (2001) Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system. J Clin Microbiol 39(3):924–929CrossRef PubMed PubMedCentral
  Wilkie IW, Harper M, Boyce JD, Adler B (2012) Pasteurella multocida: Diseases and pathogenesis. In: Aktories K, Orth JHC, Adler B (eds) Pasteurella multocida, vol 361. Springer, Berlin, pp 1–22CrossRef
  Zamri-Saad M, Ernie Z, Sabri M (2006) Protective effect following intranasal exposure of goats to live Pasteurella multocida B: 2. Trop Anim Health Prod 38(7-8):541–546CrossRef PubMed
  
• 作者单位：Hayder Hamzah Ibrahim (1) (2)
  Yusuf Abba (6)
  Ihsan Muneer Ahmed (4)
  Faez Firdaus Abdullah Jesse (1) (3)
  Eric Lim Teik Chung (1)
  Ali Dhiaa Marza (1) (5)
  Mohd Zamri-Saad (6)
  Abdul Rahman Omar (7)
  Md Zuki Abu Bakar (7)
  Abdul Aziz Saharee (1)
  Abdul Wahid Haron (1)
  Mohd Azmi Mohd Lila (6)
  
  1. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Malaysia
  2. BabilTechnical Institute, Al Furat Al-Awsat Technical University, Al Furat Al-Awsat, Iraq
  6. Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Malaysia
  4. Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq
  3. Research Centre for Ruminant Diseases, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Malaysia
  5. Department of Veterinary Internal Medicine, College of Veterinary Medicine, Al-Qasim Green University, Al-Qasim, Iraq
  7. Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Malaysia
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Pathology
  Hematology
  Oncology
  
• 出版者：Springer London
• ISSN：1618-565X

文摘

Pasteurella multocida is responsible for one of the major animal diseases with economic importance in both developed and developing countries. P. multocida B:2 causes haemorrhagic septicemia (HS) in cattle and buffaloes, which result in heavy economic losses through direct effect, resulting in high mortality rates and indirect effects through impairment of the animal productivity. It is well known that HS affects mainly the respiratory and digestive tracts of host animals. However, involvement of the reproductive system in the pathogenesis of HS has not been reported previously. This study was designed to present detailed detection and histopathological changes of the reproductive system and mammary glands of buffaloes experimentally infected with P. multocida B:2. Briefly, nine healthy buffalo calves aged 8-month-old were divided into three groups of three calves each. Calves from groups 1 and 2 were inoculated subcutaneously and orally with 10 mL of 1 × 1012 cfu/mL of P. multocida B:2, respectively, while calves of group 3 were inoculated orally with 10 mL of phosphate buffer saline as a sham control. All the calves in group 1 had to be euthanized after 12 h post-infection, while calves in group 2 were euthanized after day 21 post-infection. Successful isolation and PCR confirmation of P. multocida B:2 was achieved from different parts of the reproductive system, including ovary, oviduct, uterine horn, uterine body and vagina as well as mammary glands and supramammary lymph nodes of the buffaloes in group 1 after 12 h post-infection. However, P. multocida B:2 was not detected in the reproductive organs of buffaloes in group 2 and group 3 after 21 days post-infection. Significant differences (P < 0.05) were found in histopathological changes between the two groups, which mainly affected different anatomic regions of the reproductive system. This work provides an insight into the involvement of the female reproductive system of buffaloes during the pathogenesis of HS and shows that route of inoculation strongly affect the localization of the bacterium in the reproductive system.