Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging

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• 作者：Janina D’Alvise (1)
  Rasmus Mortensen (2)
  Steen H. Hansen (1)
  Christian Janfelt (1)
  
• 关键词：DESI ; Lidocaine ; Mass spectrometry imaging ; Pig ear skin ; Drugs ; Topical
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：406
• 期：15
• 页码：3735-3742
• 全文大小：
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• 作者单位：Janina D’Alvise (1)
  Rasmus Mortensen (2)
  Steen H. Hansen (1)
  Christian Janfelt (1)
  
  1. Department of Pharmacy, University of Copenhagen, Universitetsparken 2, Copenhagen, 2100, Denmark
  2. Pharmaceutical Technologies, LEO Pharma A/S, Industriparken 55, 2750, Ballerup, Denmark
  
• ISSN：1618-2650

文摘

Desorption electrospray ionization (DESI) mass spectrometry imaging is demonstrated as a detection technique for penetration experiments of drugs in skin. Lidocaine ointment was used as the model compound in ex vivo experiments with whole pig ears as the skin model. Follicular transport of lidocaine into the deeper skin layers is demonstrated for the first time. Furthermore, metabolism of lidocaine to 3-OH-lidocaine was observed in subcutaneous tissue as well as in lobules of white adipose tissue surrounding the hair follicles. These results suggest that it is advantageous to use full thickness skin, including subcutaneous tissue, for skin metabolism studies. Figure ?/div>