Designing a new chitinase with more chitin binding and antifungal activity

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• 作者：Soheila Matroodi (1) (2)
  Mostafa Motallebi (1)
  Mohammadreza Zamani (1)
  Mehdi Moradyar (1)
  
• 关键词：Chitinase ; Chitin binding domain ; Chimeric chitinase ; Trichoderma atroviride ; Antifungal activity
• 刊名：World Journal of Microbiology and Biotechnology
• 出版年：2013
• 出版时间：August 2013
• 年：2013
• 卷：29
• 期：8
• 页码：1517-1523
• 全文大小：381KB
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• 作者单位：Soheila Matroodi (1) (2)
  Mostafa Motallebi (1)
  Mohammadreza Zamani (1)
  Mehdi Moradyar (1)
  
  1. National Institute of Genetic Engineering and Biotechnology, P.O. Box 149651/161, Tehran, Iran
  2. Faculty of Marine Sciences, Khorramshahr Marine Sciences and Technology University, Khorramshahr, Iran
  

文摘

Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. Chitinase Chit42 from Trichoderma atroviride PTCC5220 is considered to play an important role in the biocontrol activity of this fungus against plant pathogens. Chit42 lacks a chitin binding domain (ChBD). We have produced a chimeric chitinase with stronger chitin-binding capacity by fusing to Chit42 a ChBD from Serratia marcescens Chitinase B. The fusion of ChBD improved the affinity to crystalline and colloidal chitin and also the enzyme activity of the chimeric chitinase when compared with the native Chit42. The chimeric chitinase showed higher antifungal activity toward phytopathogenic fungi.