Inhibitory effect of allicin on the growth of Babesia and Theileria equi parasites

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• 作者：Akram Ahmed Salama (1) (2)
  Mahmoud AbouLaila (3)
  Mohamad Alaa Terkawi (1)
  Ahmed Mousa (1)
  Ahmed El-Sify (2)
  Mahmoud Allaam (2)
  Ahmed Zaghawa (2)
  Naoaki Yokoyama (1)
  Ikuo Igarashi (1)
  
• 刊名：Parasitology Research
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：113
• 期：1
• 页码：275-283
• 全文大小：644 KB
• 作者单位：Akram Ahmed Salama (1) (2)
  Mahmoud AbouLaila (3)
  Mohamad Alaa Terkawi (1)
  Ahmed Mousa (1)
  Ahmed El-Sify (2)
  Mahmoud Allaam (2)
  Ahmed Zaghawa (2)
  Naoaki Yokoyama (1)
  Ikuo Igarashi (1)
  
  1. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan
  2. Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, Minoufiya, Egypt
  3. Department of Parasitology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, Minoufiya, Egypt
  
• ISSN：1432-1955

文摘

Allicin is an active ingredient of garlic that has antibacterial, antifungal, antiviral, and antiprotozoal activity. However, the inhibitory effects of allicin on Babesia parasites have not yet been examined. In the present study, allicin was tested as a potent inhibitor against the in vitro growth of bovine and equine Babesia parasites and the in vivo growth of Babesia microti in a mouse model. The in vitro growth of Babesia bovis, Babesia bigemina, Babesia caballi, or Theileria equi was inhibited by allicin in a dose-dependent manner and had IC50 values of 818, 675, 470, and 742?μM, respectively. Moreover, allicin significantly inhibited (P-lt;-.001) invasion of B. bovis, B. bigemina, B. caballi, and T. equi into the host erythrocyte. Furthermore, mice treated with 30?mg/kg of allicin for 5?days significantly (P-lt;-.05) reduced the parasitemia of B. microti over the period of the study. To further examine the potential synergism of allicin with diminazene aceturate, growth inhibitory assays were performed in vitro and in vivo. Interestingly, combinations of diminazene aceturate with allicin synergistically potentiated its inhibitory effects in vitro and in vivo. These results indicate that allicin might be beneficial for the treatment of babesiosis, particularly when used in combination with diminazene aceturate.