Genetic polymorphism among five species of Indus River carps on the basis of random amplified polymorphic DNA (RAPD) analysis

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• 作者：Haji Muhammad ; Zafar Iqbal ; Qamar Bashir
• 关键词：Genetic diversity ; RAPD ; Indus River ; Carps and gene flow
• 刊名：Rendiconti Lincei
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：28
• 期：1
• 页码：73-80
• 全文大小：
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment, general; Earth Sciences, general; Life Sciences, general; Biomedicine, general; History of Science;
• 出版者：Springer International Publishing
• ISSN：1720-0776
• 卷排序：28

文摘

The knowledge of molecular genetics is a key to understand biodiversity, evolutionary relationship, conservation programs, and genetic structure of species of an ecosystem. To study genetic polymorphism in morphological variants of five species of Indus River carps, namely, Labeo gonius, Labeo rohita, Labeo calbasu, Catla catla, and Cirrhinus mrigala, random amplified polymorphic DNA (RAPD) analysis was used. Using eight primers, a total of 719 loci were amplified comprising of 389 polymorphic loci (53.7%) and 330 monomorphic loci (45.3%). The mean value of observed number of alleles (na), effective number of alleles (ne), Nei gene diversity (h), and Shannon’s information index were 2, 1.65, 0.38, and 0.56, respectively. The mean value of Nei total gene diversity (Ht), gene diversity within the population (Hs), relative magnitude of differentiation among population (Gst), and gene flow (Nm) were 0.38, 0.26, 0.28, and 3.98, respectively. The mean value of Hs and Gst suggests moderate gene flow between these fish populations. This study revealed that C. catla is more close to L. gonius, L. rohita, and L. calbasu than C. mrigala. We have presented the first detailed study of genetic variation within and among five fish species of Indus River carps in Pakistan.