Population identification of Sarcoptes hominis and Sarcoptes canis in China using DNA sequences

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• 作者：YaE Zhao ; ZhiGuo Cao ; Juan Cheng ; Li Hu ; JunXian Ma ; YuanJun Yang…
• 关键词：Sarcoptes hominis ; Sarcoptes canis ; Population identification ; mtDNA cox1 ; mtDNA 16S ; rDNA ITS2
• 刊名：Parasitology Research
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：114
• 期：3
• 页码：1001-1010
• 全文大小：1,709 KB
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• 作者单位：YaE Zhao (1)
  ZhiGuo Cao (1) (2)
  Juan Cheng (1)
  Li Hu (1)
  JunXian Ma (1)
  YuanJun Yang (1)
  XiaoPeng Wang (3)
  JiHui Zeng (4)
  TianPing Wang (2)
  
  1. Department of Immunology and Pathogen Biology, Xi’an Jiaotong University Health Science Center, No.76 Yanta West Road, Xi’an, China, 710061
  2. Anhui Provincial Institute of Schistosomiasis Control, Hefei, China
  3. Department of Dermatology and Venereology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
  4. Xi’an PET Animal Hospital, Xi’an, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

There has been no consistent conclusion on whether Sarcoptes mites parasitizing in humans and animals are the same species. To identify Sarcoptes (S.) hominis and S. canis in China, gDNA was extracted from individual mites (five from patients with scabies and five from dogs with mange) for amplification of rDNA ITS2, mtDNA 16S, and cox1 fragment sequences. Then, the sequences obtained were aligned with those from different hosts and geographical locations retrieved from GenBank and sequence analyses were conducted. Phylogenetic trees based on 317-bp mtDNA cox1 showed five distinctive branches (species) of Sarcoptes mites, four for S. hominis (S. hominis Chinese, S. nr. hominis Chinese, S. hominis Australian, and S. hominis Panamanian) and one for S. animal (S. animal). S. animal included mites from nine animal species, with S. canis China, S. canis Australia, and S. canis USA clustering as a subbranch. Further sequence divergence analysis revealed no overlap between intraspecific (?.6?%) and interspecific (2.6-0.5?%) divergences in 317-bp mtDNA cox1. However, overlap was detected between intra- and interspecific divergences in 311-bp rDNA ITS2 or 275-bp mtDNA 16S when the divergences exceeded 1.0?%, which resulted in failure in identification of Sarcoptes. The results showed that the 317-bp mtDNA cox1 could be used as a DNA barcode for molecular identification of Sarcoptes mites. In addition, geographical isolation was observed between S. hominis Chinese, S. hominis Australian, and S. hominis Panamanian, but not between all S. canis. S. canis and the other S. animal belonged to the same species.