Paper-based ion concentration polarization device for selective preconcentration of muc1 and lamp-2 genes

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• 作者：Seok Young Son ; Hyomin Lee ; Sung Jae Kim
• 刊名：Micro and Nano Systems Letters
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：5
• 期：1
• 全文大小：1027KB
• 刊物主题：Circuits and Systems; Electrical Engineering; Mechanical Engineering; Nanotechnology; Applied and Technical Physics;
• 出版者：Springer Berlin Heidelberg
• ISSN：2213-9621
• 卷排序：5

文摘

Recently, novel biomolecules separation and detection methods based on ion concentration polarization (ICP) phenomena have been extensively researched due to its high amplification ratio and high-speed accumulation. Despite of these bright advances, the fabrication of conventional ICP devices still have complicated and times-consuming tasks. As an alternative platform, a paper have been recently used for the identical ICP operations. In this work, we demonstrated the selective preconcentration of a muc1 gene fragment as human breast cancer marker and a lamp-2 gene fragment as the cause of Danon disease in paper-based ICP devices. As a result, these two DNA fragments were successfully concentrated up to ~60 fold at different location in a single paper-channel. The device would be a promising platform for point-of-care device due to an economic fabrication, the easy extraction of concentrated sample and an easy disposability.