B ?A Conformational Transition of DNA under the Influence of a Copper Thiolate, the Copper-cysteine: Biophysical, Spectroscopic and Gel Electrophoresis Studies

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• 作者：Kalyan K. Mukherjea ; Gourisankar Panda and Sabyasachi Chatterjee
• 刊名：Transition Metal Chemistry
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：30
• 期：7
• 页码：884-888
• 全文大小：261 KB

文摘

The interaction of calf-thymus DNA with copper(II)-cysteine (Cucys) was monitored by various biophysical methods. The interaction ratio was determined by the u.v.-spectrophotometric method, which was found to be 0.14. As the DNA 260 nm band remained insensitive followed by interaction with copper(II)-cysteine, the interaction ratio and the binding constant were determined by monitoring the 248 nm copper-cysteine charge-transfer (CT) band. Visible d–d spectra of Cucys proved that copper remained in the copper(II) state after interaction with DNA. The CD and thin film i.r. spectral studies revealed that there occurred B→A conformational transition of DNA when it was bound by copper-cysteine. Gel electrophoresis study confirmed the binding pattern.