Effect of dietary supplementation of Bacillus subtilis on haematological and immunological parameters of Catla catla (Hamilton)

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• 作者：Rajesh Kumar ; Subhas C. Mukherjee ; Ritesh Ranjan ; T. Vani…
• 关键词：Bacillus subtilis ; Aeromonas hydrophila ; Catla catla ; Immunity ; Respiratory burst activity ; Total erythrocyte count
• 刊名：Aquaculture International
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：23
• 期：5
• 页码：1275-1292
• 全文大小：476 KB
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• 作者单位：Rajesh Kumar (1) (3)
  Subhas C. Mukherjee (1)
  Ritesh Ranjan (1) (4)
  T. Vani (2)
  Rajeev K. Brahmachari (1) (5)
  Sukanta K. Nayak (1) (6)
  
  1. Aquatic Animal Health Management Division, Central Institute of Fisheries Education (CIFE), Mumbai, 400 061, India
  3. Aquaculture Production and Environment Division, Central Institute of Freshwater Aquaculture, Bhubaneswar, 751 002, Odisha, India
  4. Mariculture Division, VRC of CMFRI, Visakhapatnam, 530 003, Andhra Pradesh, India
  2. Inland Aquaculture Division, CIFE, Mumbai, 400 061, India
  5. Department of Fish Microbiology, College of Fisheries, Dholi, Muzaffarpur, 843 121, Bihar, India
  6. Department of Biotechnology, North Orissa University, Baripada, Odisha, India
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  
• 出版者：Springer Netherlands
• ISSN：1573-143X

文摘

Effect of dietary supplementation of a Gram-positive, aerobic, probiotic bacterium Bacillus subtilis on the immunohaematological indices during pre- and post-challenge in Indian major carp, catla (Catla catla), was studied. The B. subtilis was administered orally at four different doses 1.0 × 106 (T₁), 1.0 × 107 (T₂), 1.0 × 108 (T₃), and 1.0 × 109 (T₄) cfu g? feed to C. catla for 90 days. The positive control (C_p) and negative control (C_n) were fed with feed without B. subtilis for the same period. On the 60th day, blood and serum were sampled to determine various haematological and serum parameters. Fish were challenged intraperitoneally with Aeromonas hydrophila after 60 days in all the treatment groups and C_p, while the C_n was challenged with phosphate-buffered saline (PBS, pH 7.2) only. Dietary supplementation of B. subtilis leads to the rise of various immunological and haematological parameters in catla during the pre- and post-challenge. During pre-challenge, the highest TEC (1.30 ± 0.02 × 106 cells mm?), haemoglobin (7.43 ± 0.25 g %), total serum protein (3.89 ± 0.08 g dL?), and serum lysozyme activity (8.39 ± 0.01 μg ml?) were recorded in fishes fed feed containing B. subtilis at 1 × 109 cfu/g feed (T₄). The highest survival percentage (86.33 %) was also observed in T₄ group. The significantly increased survival percentage (P < 0.05) of B. subtilis-treated groups in comparison with control group (C_p) suggests that dietary supplementation of this probiotic bacterium can protect catla from A. hydrophila infection by enhancing innate immunity. Keywords Bacillus subtilis Aeromonas hydrophila Catla catla Immunity Respiratory burst activity Total erythrocyte count