Identification of beta cell dysfunction at the pre-symptomatic stage of diabetes mellitus by novel analytical system: liquid biopsy measurements in femtograms
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- 作者：Kurt Krapfenbauer
- 关键词：Biochip ; Clinical applications ; Miniaturization ; Multiplexing ; Personalized and predictive medicine ; Novel detection conjugate ; Diabetes mellitus ; Pancreatic polypeptide ; Beta cell failure
- 刊名：EPMA Journal
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：8
- 期：1
- 全文大小：638KB
- 刊物主题：Biomedicine, general; Medicine/Public Health, general;
- 出版者：BioMed Central
- ISSN：1878-5085
- 卷排序：8
文摘
BackgroundDiabetes mellitus is produced and progresses as a consequence of complex and gradual processes, in which a variety of alterations of the endocrine pancreas, are involved and which mainly result in beta cell failure. Those molecular alterations can be found in the bloodstream, which suggests that we could quantify specific biomarkers in plasma or serum by very sensitive methods before the onset diabetes mellitus is diagnosed. However, classical methods of protein analysis such as electrophoresis, Western blot, ELISA, and liquid chromatography are generally time-consuming, lab-intensive, and not sensitive enough to detect such alteration in a pre-symptomatic state of the disease.