Detection of extremely low concentration waterborne pathogen using a multiplexing self-referencing SERS microfluidic biosensor

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• 作者：Chao Wang ; Foram Madiyar ; Chenxu Yu ; Jun Li
• 刊名：Journal of Biological Engineering
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：11
• 期：1
• 全文大小：2552KB
• 刊物类别：Engineering
• 刊物主题：Biomedical Engineering; Biotechnology; Biological Techniques; Nucleic Acid Chemistry; Applied Microbiology; Environmental Engineering/Biotechnology;
• 出版者：BioMed Central
• ISSN：1754-1611
• 卷排序：11

文摘

BackgroundIt is challenging to achieve ultrasensitive and selective detection of waterborne pathogens at extremely low levels (i.e., single cell/mL) using conventional methods. Even with molecular methods such as ELISA or PCR, multi-enrichment steps are needed which are labor and cost intensive. In this study, we incorporated nano-dielectrophoretic microfluidic device with Surface enhanced Raman scattering (SERS) technique to build a novel portable biosensor for easy detection and characterization of Escherichia coli O157:H7 at high sensitivity level (single cell/mL).