Comparison of the alendronate and irradiation with a light-emitting diode (LED) on murine osteoclastogenesis
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- 作者：Hong Moon Sohn ; Youngjong Ko ; Mineon Park ; Bora Kim…
- 关键词：Osteoporosis ; LED ; ROS ; Osteoclastogenesis
- 刊名：Lasers in Medical Science
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：32
- 期：1
- 页码：189-200
- 全文大小：
- 刊物类别：Medicine
- 刊物主题：Medicine/Public Health, general; Dentistry; Optics, Lasers, Photonics, Optical Devices; Quantum Optics;
- 出版者：Springer London
- ISSN：1435-604X
- 卷排序：32
文摘
Photomodulation therapy (PBMT) using light-emitting diode (LED) has been proposed as an alternative to conventional osteoporosis therapies. Our aim was to determine the effect of irradiation with a light-emitting diode on receptor activator of NF-κB ligand (RANKL)-mediated differentiation of mouse bone marrow macrophages into osteoclasts and compare it to alendronate treatment. The cells were irradiated with LED at 635±10 nm, 9-cm spot size, 5 mW/cm2, and 18 J for 60 min/day in a CO2 incubator. The differentiation of irradiated and untreated RANKL-stimulated bone marrow macrophages into osteoclasts was evaluated by tartrate-resistant acid phosphatase (TRAP) staining and by molecular methods. These included assessing messenger RNA (mRNA) expression of osteoclastic markers such as TRAP, c-Fos, Atp6v0d2, DC-STAMP, NFATc1, cathepsin K, MMP9 and OSCAR; phosphorylation of various MAPKs, including extracellular signal-regulated kinase ERK1/2, P38, and JNK; NF-κB translocation; and resorption pit formation. Results were compared to those obtained with sodium alendronate. Production of reactive oxygen species was measured by a 2’,7’-dihydrodichlorofluorescein diacetate assay. LED irradiation and alendronate inhibited mRNA expression of osteoclast-related genes, such as TRAP, c-Fos, and NFATc1, and reduced the osteoclast activity of RANKL-stimulated bone marrow macrophages. LED irradiation, but not alendronate, also inhibited the production of reactive oxygen species (ROS); phosphorylation of ERK, P38, and IκB; and NF-κB translocation. These findings suggest that LED irradiation downregulates osteoclastogenesis by ROS production; this effect could lead to reduced bone loss and may offer a new therapeutic tool for managing osteoporosis.