Structural Analysis of the Lipopeptide Produced by the Bacillus subtilis Mutant R2-104 with Mutagenesis

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• 作者：Yong Meng ; Wei Zhao ; Jia You ; Hong-Ze Gang…
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：179
• 期：6
• 页码：973-985
• 全文大小：818 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291
• 卷排序：179

文摘

The lipopeptide and its homologues are a kind of the five major biosurfactants with prominent interfacial and biological activities. A suite of mutagenesis method was adopted to expose a wild lipopeptide-producing strain Bacillus subtilis HSO121 to improve lipopeptide yield, and a stable mutant named R2-104 with a 2.0-fold production of lipopeptide was obtained. Compared to that of the wild strain HSO121, the lipopeptide produced by R2-104 showed a similar surface activity, but the course profiles of lipopeptide production during cultivation were different, with the peak yield of 500 mg at about 9 h by R2-104, and 400 mg at about 5 h by HSO121. The constituent abundance of the lipopeptide homologues produced by R2-104 was also different from that by HSO121. Combined methods of ESI-MS, GC-MS and MS-MS were applied for structural characterization of lipopeptide homologues, and it showed that the lipopeptides produced by R2-104 and HSO121 were attributed to a surfactin family with different constituents. The dominant constituent of the surfactin family produced by R2-104 was anteiso C₁₅-surfactin with a relative content of 43.8 %, while the dominant one produced by HSO121was iso C₁₄-surfactin with a relative content of 33.1 %.KeywordsMutagenesisBacillus subtilisLipopeptideSurfactinStructural analysis