Enhanced production of reducing sugars from transgenic rice expressing exo-glucanase under the control of a senescence-inducible promoter
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- 作者：Kayoko Furukawa (1)
Shin Ichikawa (1)
Mutsumi Nigorikawa (1)
Tomonori Sonoki (2)
Yukihiro Ito (1) - 关键词：Saccharification ; Cell wall ; Cellulase ; Senescence ; Biofuel ; Rice
- 刊名：Transgenic Research
- 出版年：2014
- 出版时间：June 2014
- 年：2014
- 卷：23
- 期：3
- 页码：531-537
- 全文大小：
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Shin Ichikawa (1)
Mutsumi Nigorikawa (1)
Tomonori Sonoki (2)
Yukihiro Ito (1)
1. Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan
2. Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho, Hirosaki, 036-8560, Japan - ISSN：1573-9368
文摘
We generated transgenic rice plants that express EXG1 exo-glucanase under the control of a senescence-inducible promoter. When a GUS coding sequence was connected to a promoter region of STAY GREEN (SGR) gene of rice and introduced into rice, GUS activity was specifically observed along with senescence. When an EXG1 cDNA was connected to the SGR promoter and introduced into rice, higher cellulase activities were detected after senescence. The EXG1 transgenic plants showed enhanced enzymatic saccharification efficiencies after senescence, but no significant difference of saccharification efficiencies was observed before senescence. The saccharification efficiencies were correlated with the cellulase activities in the transgenic plants. The EXG1 transgenic plants showed neither morphological abnormality nor sterility, both of which were observed when EXG1 was constitutively overexpressed. These results indicate that expression of cell wall degrading enzymes such as cellulase by a senescence-inducible promoter is one of the ways to enhance the saccharification ability of cellulosic biomass without affecting plant growth for efficient production of biofuels.