Hydrodynamically stable adhesion of endothelial cells onto a polypropylene hollow fiber membrane by modification with adhesive protein

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• 作者：Mutsumi Takagi ; Kouji Shiwaku ; Takeomi Inoue ; Yukitoshi Shirakawa ; Yoshiki Sawa ; Hikaru Matsuda and Toshiomi Yoshida
• 关键词：Adhesion protein ; Endothelial cell adhesion ; Hybrid artificial lung ; Fibronectin ; Polypropylene hollow fiber
• 刊名：Journal of Artificial Organs
• 出版年：2003
• 出版时间：September 2003
• 年：2003
• 卷：6
• 期：3
• 页码：222-226
• 全文大小：232 KB
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Surgery
  Cardiac Surgery
  Biomedical Engineering
  Orthopedics
  Nephrology
  Hepatology
  
• 出版者：Springer Japan
• ISSN：1619-0904

文摘

The effect on the adhesion of endothelial cells of immobilization of adhesion proteins onto a microporous polypropylene hollow fiber membrane for a conventional artificial lung was investigated with the aim of constructing a hybrid artificial lung bearing endothelial cells on the modified membrane. The membrane was modified by adsorption or covalent bonding of adhesion proteins of fibronectin, gelatin, or Pronectin. The density of adherent cells on the membrane modified by adsorption of or covalent bonding with fibronectin reached 1 × 105 cells/cm2 after 1 day of incubation, which corresponds to the confluent cell density in a conventional culture dish, while the cell densities on the membranes modifieds with gelatin and Pronectin were 1–5 × 104 cells/cm2 and 0.5–1 × 104 cells/cm2, respectively. The loading of hydrodynamic shear force (0.23N/m2) for 30min to the membranes bearing endothelial cells had little effect on the density of adhered cells. The membrane covalently bonded with fibronectin could well maintain a high cell density even after the loading of a higher shear force of 1.15N/m2 for 180min, however, at this level of shear force 49% of adhered cells on the fibronectin-adsorbed membrane were lost after 30min. A partial cardiopulmonary bypass in rats employing the hybrid artificial lung model composed of a polypropylene hollow fiber membrane covalently bonded with fibronectin and endothelial cell adhesion showed the inhibition of tumor necrosis factor- release and an increase in IL-10 concentration in the circulating blood compared with that employing an artificial lung without cells. Long-term partial cardiopulmonary bypass employing the hybrid artificial lung model should be studied further.