Multiplex PCR as a novel method in the diagnosis of spinal tuberculosis–a pilot study

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• 作者：Kusum Sharma ; Rajesh Kumar Meena ; Ashish Aggarwal ; Rajesh Chhabra
• 关键词：Multiplex PCR ; Spine ; AFB smear ; Tuberculosis
• 刊名：Acta Neurochirurgica
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：159
• 期：3
• 页码：503-507
• 全文大小：
• 刊物主题：Neurosurgery; Interventional Radiology; Neuroradiology; Neurology; Surgical Orthopedics; Minimally Invasive Surgery;
• 出版者：Springer Vienna
• ISSN：0942-0940
• 卷排序：159

文摘

BackgroundEstablishment of a reliable and rapid diagnosis is of paramount importance in spinal tuberculosis. The available gadgetry of investigations, such as AFB smear, culture of Mycobacterium tuberculosis, and Uniplex PCR, suffers from a lack of adequate sensitivity and/or a lack of rapidity. Therefore, many times a diagnosis is made either very late in the disease process or sometimes empirical therapy has to be started because a definite diagnosis could not be made. All of these are not ideal situations for a clinician. The present study was done with the aim to establish a rapid and reliable diagnosis of M. tuberculosis infection. This was established by identifying M. tuberculosis genes.