Colonization and molecular diversity of arbuscular mycorrhizal fungi associated with the rhizosphere of cowpea (Vigna unguiculata (L.) Walp.) in Benin (West Africa): an exploratory study

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• 作者：Jean-Martial Johnson ; Pascal Houngnandan ; Aboubacry Kane…
• 关键词：AMF diversity ; Arbuscular mycorrhizas ; Operational taxonomic unit ; Rhizosphere ; Vigna unguiculata
• 刊名：Annals of Microbiology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：66
• 期：1
• 页码：207-221
• 全文大小：751 KB
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• 作者单位：Jean-Martial Johnson (1) (2)
  Pascal Houngnandan (1)
  Aboubacry Kane (3)
  Odile Chatagnier (4)
  Kadidia B. Sanon (5)
  Marc Neyra (6) (7)
  Diederik van Tuinen (4)
  
  1. Laboratoire d’Ecologie Microbienne, Faculté des Sciences Agronomiques, Université d’Abomey-Calavi (UAC), Campus d’Abomey-Calavi, 01 BP 526, Cotonou, Bénin
  2. Africa Rice Center (AfricaRice), 01 BP 2031, Cotonou, Benin
  3. Laboratoire Commun de Microbiologie (LCM), IRD/UCAD/ISRA, Centre de Recherche de Bel Air, BP 1386, 18524, Dakar, Sénégal
  4. UMR 1347 Agroécologie, Pôle Interactions Plantes-Microorganismes, CNRS ERL 6300, INRA/Agrosup/Université de Bourgogne, 17 rue Sully, BP 86510, 21065, Dijon Cedex, France
  5. Laboratoire de Microbiologie, Département de Productions Forestières, Institut de l’Environnement et des Recherches Agricoles (INERA), 03 BP 7047, Ouagadougou 03, Burkina-Faso
  6. UR MALY, Irstea, Centre de Lyon-Villeurbanne, 69626, Villeurbanne, France
  7. IRD-Cirad-SupAgro-UM2/USC INRA, UMR113, LSTM, 34398, Montpellier, Cedex 5, France
  
• 刊物主题：Microbiology; Microbial Genetics and Genomics; Microbial Ecology; Fungus Genetics; Medical Microbiology; Applied Microbiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-2044

文摘

Arbuscular mycorrhizal symbiosis is an important plant root–fungal partnership/interaction that affects the growth response of crops. We have investigated the molecular diversity of arbuscular mycorrhizal fungi (AMF) colonizing cowpea roots and the associated rhizosphere soil to test the hypothesis that community diversity in rhizosphere soil is similar to that in cowpea (Vigna unguiculata) roots. Cowpea plants were grown in farmers’ fields located in seven agro-ecological zones of Benin, and soil and root samples were collected. The molecular diversity of the AMF in these samples was assessed after amplification of the large ribosomal subunit of DNA extracted from the soil and the root samples. At fruition, the frequency of mycorrhizal infection was unaffected by the agro-ecological zone, but there were significant differences in the intensity of AMF colonization among the zones. Multiple regression analysis showed that the main factor affecting mycorrhizal frequency at flowering was available phosphorus. Phylogenetic analysis revealed 25 operational taxonomic units belonging to two fungal families (Glomeraceae and Gigasporaceae). The diversity of AMF colonizing roots of cowpea in Benin was high and fairly similar to that in the rhizosphere soil but with a prevalence of the Glomeraceae. Despite the absence of strict host specificity in mycorrhizal symbiosis, there was a preferential association between some AMF species and cowpea cultivar IT96D-610. Keywords AMF diversity Arbuscular mycorrhizas Operational taxonomic unit Rhizosphere Vigna unguiculata