Tracing the metabolism of HT-2 toxin and T-2 toxin in barley by isotope-assisted untargeted screening and quantitative LC-HRMS analysis

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• 作者：Jacqueline Meng-Reiterer ; Elisabeth Varga…
• 关键词：Untargeted metabolite profiling ; Stable isotopic labelling ; Fast polarity switching ; Type A trichothecenes ; Masked mycotoxins
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：407
• 期：26
• 页码：8019-8033
• 全文大小：1,173 KB
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• 作者单位：Jacqueline Meng-Reiterer (1) (2)
  Elisabeth Varga (1) (3)
  Alexis V. Nathanail (4)
  Christoph Bueschl (1)
  Justyna Rechthaler (5)
  Susan P. McCormick (6)
  Herbert Michlmayr (7)
  Alexandra Malachová (1) (3)
  Philipp Fruhmann (7) (8)
  Gerhard Adam (7)
  Franz Berthiller (1) (3)
  Marc Lemmens (2)
  Rainer Schuhmacher (1)
  
  1. Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Str. 20, 3430, Tulln, Austria
  2. Institute for Biotechnology in Plant Production, IFA-Tulln, BOKU, Konrad-Lorenz-Str. 20, 3430, Tulln, Austria
  3. Christian Doppler Laboratory for Mycotoxin Metabolism, IFA-Tulln, BOKU, Konrad-Lorenz-Str. 20, 3430, Tulln, Austria
  4. Chemistry and Toxicology Unit, Research and Laboratory Department, Finnish Food Safety Authority (Evira), Mustialankatu 3, 00790, Helsinki, Finland
  5. University of Applied Sciences Wr. Neustadt, Degree Programme Biotechnical Processes (FHWN-Tulln), Konrad-Lorenz-Str. 10, 3430, Tulln, Austria
  6. Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, 1815 N. University Street, Peoria, IL, 61604, USA
  7. Department of Applied Genetics and Cell Biology, BOKU, Konrad-Lorenz-Str. 24, 3430, Tulln, Austria
  8. Institute of Applied Synthetic Chemistry, Vienna University of Technology, Getreidemarkt 9/163, 1060, Vienna, Austria
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

An extensive study of the metabolism of the type A trichothecene mycotoxins HT-2 toxin and T-2 toxin in barley using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) is reported. A recently developed untargeted approach based on stable isotopic labelling, LC-Orbitrap-MS analysis with fast polarity switching and data processing by MetExtract software was combined with targeted LC-Q-TOF-MS(/MS) analysis for metabolite structure elucidation and quantification. In total, 9 HT-2 toxin and 13 T-2 toxin metabolites plus tentative isomers were detected, which were successfully annotated by calculation of elemental formulas and further LC-HRMS/MS measurements as well as partly identified with authentic standards. As a result, glucosylated forms of the toxins, malonylglucosides, and acetyl and feruloyl conjugates were elucidated. Additionally, time courses of metabolite formation and mass balances were established. For absolute quantification of those compounds for which standards were available, the method was validated by determining apparent recovery, signal suppression, or enhancement and extraction recovery. Most importantly, T-2 toxin was rapidly metabolised to HT-2 toxin and for both parent toxins HT-2 toxin-3-O-β-glucoside was identified (confirmed by authentic standard) as the main metabolite, which reached its maximum already 1 day after toxin treatment.