Attenuated platelet aggregation in patients with septic shock is independent from the activity state of myosin light chain phosphorylation or a reduction in Rho kinase-dependent inhibition of myosin light chain phosphatase

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• 作者：Benjamin AJ Reddi ; Samantha M Iannella…
• 关键词：Platelets ; Sepsis ; Shock ; Aggregation ; Rho kinase ; Myosin phosphatase ; Whole blood impedance aggregometry ; Illness severity score
• 刊名：Intensive Care Medicine Experimental
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：3
• 期：1
• 全文大小：1592KB
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• 作者单位：Benjamin AJ Reddi (1) (2) (3)
  Samantha M Iannella (3)
  Stephanie N O鈥機onnor (1) (3)
  Adam M Deane (1) (3)
  Scott R Willoughby (4)
  David P Wilson (2)
  
  1. Intensive Care Unit, Royal Adelaide Hospital, North Terrace, Adelaide, South Australia, 5000, Australia
  2. Molecular Physiology of Vascular Function Laboratory, School of Medical Sciences, University of Adelaide, Adelaide, South Australia, 5000, Australia
  3. Discipline of Acute Care Medicine, University of Adelaide, Adelaide, South Australia, 5000, Australia
  4. Centre for Heart Rhythm Disorders, School of Medicine, University of Adelaide, Adelaide, South Australia, 5000, Australia
  
• 刊物类别：Intensive / Critical Care Medicine;
• 刊物主题：Intensive / Critical Care Medicine;
• 出版者：Springer International Publishing
• ISSN：2197-425X

文摘

Background Impaired coagulation contributes to the morbidity and mortality associated with septic shock. Whether abnormal platelet contraction adds to the bleeding tendency is unknown. Platelets contract when Ca2+-dependent myosin light chain kinase (MLCK) phosphorylates Ser19 of myosin light chain (MLC₂₀), promoting actin-myosin cross-bridge cycling. Contraction is opposed when myosin light chain phosphatase (MLCP) dephosphorylates MLC₂₀. It is thought that Rho kinase (ROK) inhibits MLCP by phosphorylating Thr855 of the regulatory subunit MYPT, favouring platelet contraction. This study tested the hypotheses that in septic shock, (i) platelet function is inversely correlated with illness severity and (ii) ROK-dependent MLCP inhibition and myosin light chain phosphorylation are reduced.