High-frequency plant regeneration from immature zygotic embryo cultures of Houttuynia cordata Thunb via somatic embryogenesis

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• 作者：Myung Jin Oh (1)
  Myung Suk Ahn (1)
  Eun Yee Jie (1)
  Jang Ryol Liu (1)
  Byung Whan Min (3)
  Suk Weon Kim (2)
  
• 关键词：Houttuynia cordata ; Plant regeneration ; Somatic embryogenesis ; Immature zygotic embryo cultures
• 刊名：Plant Biotechnology Reports
• 出版年：2013
• 出版时间：October 2013
• 年：2013
• 卷：7
• 期：4
• 页码：527-534
• 全文大小：
• 作者单位：Myung Jin Oh (1)
  Myung Suk Ahn (1)
  Eun Yee Jie (1)
  Jang Ryol Liu (1)
  Byung Whan Min (3)
  Suk Weon Kim (2)
  
  1. GreenBio Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahagno, Yuseong-gu, Daejeon, 30506, South Korea
  3. School of Ecological and Environmental System, Kyungpook National University, Sangju, 742-711, South Korea
  2. Microbiological Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahagno, Yuseong-gu, Daejeon, 305-806, South Korea
  
• ISSN：1863-5474

文摘

This study reports high-frequency plant regeneration from immature zygotic embryo cultures of Houttuynia cordata Thunb via somatic embryogenesis. Numerous green globular structures were directly formed on the surfaces of cotyledons and radicles from 2-week-old immature zygotic embryos at a frequency of 42.1% when cultured on Murashige and Skoog (MS) medium supplemented with 2mgl1 of naphthaleneacetic acid (NAA) and 1mgl1 of 6-benzyladenine (BA). In comparison, white globular structures and pale-yellow calluses were formed simultaneously at a frequency of 28.3% when cultured on MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). The pale-yellow calluses were transferred to MS liquid medium supplemented with 2,4-D to establish embryogenic cell suspension cultures consisting of round, isodiametric cells that formed cell aggregates. Upon plating of these cell aggregates on half-strength MS medium without growth regulators under light conditions, cell aggregates gave rise to numerous globular embryos at a frequency of 56%. Of the globular embryos, 15 % were successfully converted into cotyledonary embryos when cultured on half-strength MS medium under light conditions. The plant regeneration system of H. cordata established in this study will be useful for the selection, genetic transformation, and mass proliferation of elite clones with medicinal potential.