Analysis of the population genetics of Opisthorchis viverrini sensu lato in the Nam Ngum River wetland, Lao PDR, by multilocus enzyme electrophoresis
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- 作者：Nadda Kiatsopit (1) (2)
Paiboon Sithithaworn (1) (2)
Weerachai Saijuntha (3)
Opal Pitaksakulrat (1) (2)
Trevor N. Petney (4)
Joanne P. Webster (5)
Ross H. Andrews (1) (2) (5) - 关键词：Population genetics ; Opisthorchis viverrini ; Lao PDR ; Multilocus enzyme electrophoresis
- 刊名：Parasitology Research
- 出版年：2014
- 出版时间：August 2014
- 年：2014
- 卷：113
- 期：8
- 页码：2973-2981
- 全文大小：381 KB
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Paiboon Sithithaworn (1) (2)
Weerachai Saijuntha (3)
Opal Pitaksakulrat (1) (2)
Trevor N. Petney (4)
Joanne P. Webster (5)
Ross H. Andrews (1) (2) (5)
1. Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
2. Liver Fluke and Cholangiocarcinoma Research Center (LFCRC), Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
3. Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University, Mahasarakham, 44150, Thailand
4. Department of Ecology and Parasitology, University of Karlsruhe, Kornblumenstrasse 13, Karlsruhe, 76131, Germany
5. Imperial College London, Faculty of Medicine, St Mary鈥檚 Campus, South Wharf Street, London, W2 1NY, UK - ISSN：1432-1955
文摘
A previous population genetics study of Opisthorchis viverrini from a locality in an endemic area in Thailand found little genetic variation over time and second intermediate fish host species. Since a similar comparative analysis is not available for Lao PDR, we conducted a study of O. viverrini from different endemic foci in Vientiane Province, Lao PDR, based on spatial, temporal and fish host species. A total of 620 adult O. viverrini originating from the Nam Ngum River wetland were analysed at five previously defined polymorphic enzyme loci. Of these worms, 252 were from six different localities (spatial samples), 162 worms from different years (temporal samples) and 206 worms from four different cyprinid fish species. Significant heterozygote deficiency was found in most O. viverrini populations with levels of genetic differentiation ranging between F ST 0.0000 and 0.0197 suggesting that gene flow occurred at a variable rate. The role of temporal factors and fish host species had little influence on the level of genetic differentiation. As for O. viverrini from Thailand, these findings indicate that self-fertilization and/or a clonal distribution of O. viverrini occurs in Lao PDR. Unlike the results for O. viverrini from Thailand, spatial population substructuring may be the underlying population processes for O. viverrini in Lao PDR. These findings indicate that geographical variation may contribute to the transmission dynamics of the parasite with implications for parasite control. However, other host factors, such as snail intermediate hosts and mammal reservoir hosts, as well as human beings, may also play significant roles.