Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting

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• 作者：Nadeem Akhtar Abbasi (1)
  Tariq Pervaiz (1)
  Ishfaq Ahmed Hafiz (1)
  Mehwish Yaseen (1)
  Azhar Hussain (1)
  
• 关键词：Eriobotrya japonica ; Micropropagation ; Sterilization of loquat ; Plant growth regulator ; Shoot proliferation ; Rhizogenesis ; S661.9
• 刊名：Journal of Zhejiang University SCIENCE B
• 出版年：2013
• 出版时间：September 2013
• 年：2013
• 卷：14
• 期：9
• 页码：774-784
• 全文大小：1329KB
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• 作者单位：Nadeem Akhtar Abbasi (1)
  Tariq Pervaiz (1)
  Ishfaq Ahmed Hafiz (1)
  Mehwish Yaseen (1)
  Azhar Hussain (1)
  
  1. Plant Tissue Culture Laboratory, Department of Horticulture, Pir Mehr Ali Shah-Arid Agriculture University, Rawalpindi, 46300, Pakistan
  
• ISSN：1862-1783

文摘

In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl (5%, 7%, 10%, 12%, 14% (v/v)) for 12 min and HgCl2 (0.01%, 0.05%, 0.10%, 0.20%, 0.25% (w/v)) for 2 min. A maximum survival rate of 70% was recorded after surface sterilization with 10% NaOCl. Caulogenic response was assessed on Murashige and Skoog (MS) medium fortified with assorted combinations of the cytokinins, benzylaminopurine (BAP), kinetin, and N6-(2-isopentyl)adenine (2iP). Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots, while the highest shoot length was yielded by the combination of BAP 0.5 mg/L, kinetin 0.5 mg/L, and 2iP 3 mg/L. Higher levels of cytokinins induced callogenesis, vitrification and stunted growth to some extent. For rhizogenesis, uniform sized micro-shoots were excised and transferred to half-strength MS medium containing auxins. The best rooting expression was observed with naphthaleneacetic acid (NAA) 1 mg/L combined with indole-3-butyric acid (IBA) 2 mg/L and paclobutrazol (PBZ) 1 mg/L.