Choices for Induction of Pluripotency: Recent Developments in Human Induced Pluripotent Stem Cell Reprogramming Strategies

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• 作者：Marinka Brouwer ; Huiqing Zhou ; Nael Nadif Kasri
• 关键词：Human induced pluripotent stem cells ; Reprogramming
• 刊名：Stem Cell Reviews and Reports
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：12
• 期：1
• 页码：54-72
• 全文大小：854 KB
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• 作者单位：Marinka Brouwer (1)
  Huiqing Zhou (2) (4)
  Nael Nadif Kasri (1) (2) (3)
  
  1. Department of Cognitive Neuroscience, Radboudumc, Nijmegen, 6500, HB, The Netherlands
  2. Department of Human Genetics, Radboudumc, Nijmegen, 6500, HB, The Netherlands
  4. Department of Molecular Developmental Biology, Faculty of Science, Radboud University, Nijmegen, 6500, HB, The Netherlands
  3. Donders Institute for Brain, Cognition, and Behaviour, Centre for Neuroscience, Nijmegen, 6525, AJ, The Netherlands
  
• 刊物主题：Cell Biology;
• 出版者：Springer US
• ISSN：1558-6804

文摘

The ability to generate human induced pluripotent stem cells (iPSCs) from somatic cells provides tremendous promises for regenerative medicine and its use has widely increased over recent years. However, reprogramming efficiencies remain low and chromosomal instability and tumorigenic potential are concerns in the use of iPSCs, especially in clinical settings. Therefore, reprogramming methods have been under development to generate safer iPSCs with higher efficiency and better quality. Developments have mainly focused on the somatic cell source, the cocktail of reprogramming factors, the delivery method used to introduce reprogramming factors and culture conditions to maintain the generated iPSCs. This review discusses the developments on these topics and briefly discusses pros and cons of iPSCs in comparison with human embryonic stem cells generated from somatic cell nuclear transfer. Keywords Human induced pluripotent stem cells Reprogramming