A Novel Animal Model of Dysphagia Following Stroke

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• 作者：Naoto Sugiyama (1)
  Eiji Nishiyama (2)
  Yukitoshi Nishikawa (3)
  Takashi Sasamura (3)
  Shinji Nakade (3)
  Katsumasa Okawa (4)
  Tadashi Nagasawa (5)
  Akane Yuki (6)
  
• 关键词：Transient middle cerebral artery occlusion ; Cerebral ischemia ; Stroke ; Dysphagia ; Deglutition ; Deglutition disorders
• 刊名：Dysphagia
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：29
• 期：1
• 页码：61-67
• 全文大小：246 KB
• 作者单位：Naoto Sugiyama (1)
  Eiji Nishiyama (2)
  Yukitoshi Nishikawa (3)
  Takashi Sasamura (3)
  Shinji Nakade (3)
  Katsumasa Okawa (4)
  Tadashi Nagasawa (5)
  Akane Yuki (6)
  
  1. Food Technology Section, Industrial Research Institute of Shizuoka Prefecture, 2078 Makigaya, Aoi-ku, Shizuoka-shi, Shizuoka, 421-1298, Japan
  2. Project Management, Translational Medicine Center, Ono Pharmaceutical Co., Ltd., 3-1-1 Sakurai, Shimamoto-cho, Mishima-gun, Osaka, 618-8585, Japan
  3. Discovery Research Laboratories I, Minase Research Institute, Ono Pharmaceutical Co., Ltd., 3-1-1 Sakurai, Shimamoto-cho, Mishima-gun, Osaka, 618-8585, Japan
  4. Planning Department, Industrial Research Institute of Shizuoka Prefecture, 2078 Makigaya, Aoi-ku, Shizuoka-shi, Shizuoka, 421-1298, Japan
  5. Electronic Technology Section, Industrial Research Institute of Shizuoka Prefecture, 2078 Makigaya, Aoi-ku, Shizuoka-shi, Shizuoka, 421-1298, Japan
  6. Environment Technology Section, Industrial Research Institute of Shizuoka Prefecture, 2078 Makigaya, Aoi-ku, Shizuoka-shi, Shizuoka, 421-1298, Japan
  
• ISSN：1432-0460

文摘

Patients who have an ischemic stroke are at high risk of swallowing disorders. Aspiration due to swallowing disorders, specifically delayed trigger of the pharyngeal stage of swallowing, predisposes such patients to pneumonia. In the present study, we evaluated swallowing reflex in a rat model of transient middle cerebral artery occlusion (tMCAO), which is one of the most common experimental animal models of cerebral ischemia, in order to develop a novel animal model of dysphagia following ischemic stroke. A swallowing reflex was elicited by a 10-s infusion of distilled water (DW) to the pharyngolaryngeal region in the tMCAO rat model. Swallowing reflex was estimated using the electromyographic activity of the mylohyoid muscle from 1 to 3?weeks after surgery. Two weeks after tMCAO, the number of swallows significantly decreased and the onset latency of the first swallow was prolonged compared with that of the sham group. The number of swallows in rats significantly increased by infusions of 10?mM citric acid and 0.6?μM capsaicin to the pharyngolaryngeal region compared with the number from infusion of DW. It has been reported that sensory stimulation of the pharyngolaryngeal region with citric acid, capsaicin, and l-menthol ameliorates hypofunction of pharyngeal-stage swallowing in dysphagia patients. Therefore, the tMCAO rat model may show some of the symptoms of pharyngeal-stage swallowing disorders, similar to those in patients with ischemic stroke. This rat tMCAO model has the potential to become a novel animal model of dysphagia following stroke that is useful for development of therapeutic methods and drugs.