The decreased secretion of hyaluronan by older human fibroblasts under physiological conditions is mainly associated with the down-regulated expression of hyaluronan synthases but not with the expression levels of hyaluronidases

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• 作者：Shuko Terazawa ; Hiroaki Nakajima ; Keisuke Tobita ; Genji Imokawa
• 关键词：Human dermal fibroblasts ; Hyaluronan ; Hyaluronan synthase ; Hyaluronidase ; Ultraviolet B
• 刊名：Cytotechnology
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：67
• 期：4
• 页码：609-620
• 全文大小：1,697 KB
• 参考文献：Averbeck M, Beilharz S, Bauer M, Gerbhardt C, Hartmann A, Hochleitner K, Kauer F, Voith U, Simon JC, Termeer C (2006) In situ profiling and quantification of cytokines released during ultraviolet B-induced inflammation by combing dermal microdialysis and protein microarrays. Exp Dermatol 15:447-54View Article
  Dai G, Freudenberger T, Zipper P, Melchior A, Grether-Beck S, Rabausch B, de Groot J, Twarock S, Hanenberg H, Homey B, Krutmann J, Reifenberger J, Fischer JW (2007) Chronic ultraviolet B irradiation causes loss of hyaluronic acid from mouse dermis because of down-regulation of hyaluronic acid synthases. Am J Pathol 171:1451-461View Article
  Elias JA, Krol RC, Freundlich B, Sampson PM (1988) Regulation of human lung fibroblast glycosaminoglycan production by recombinant interferons, tumor necrosis factor, and lymphotoxin. J Clin Invest 81:325-33View Article
  Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S, Voorhees JJ (1996) Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature 379:335-39View Article
  Fisher GJ, Varani J, Voorhees JJ (2008) Looking older: fibroblast collapse and therapeutic implications. Arch Dermatol 144:666-72View Article
  Fisher GJ, Quan T, Purohit T, Shao Y, Cho MK, He T, Varani J, Kang S, Voorhees JJ (2009) Collagen fragmentation promotes oxidative stress and elevates matrix metalloproteinase-1 in fibroblasts in aged human skin. Am J Pathol 174:101-14View Article
  Hata R, Sunada H, Arai K, Sato T, Ninomiya Y, Nagai Y, Senoo H (1988) Regulation of collagen metabolism and cell growth by epidermal growth factor and ascorbate in cultured human skin fibroblasts. Eur J Biochem 173:261-67View Article
  Heldin P, Laurent TC, Heldin CH (1989) Effect of growth factors on hyaluronan synthesis in cultured human fibroblasts. Biochem J 258:919-22
  Itano N, Kimata K (1996) Molecular cloning of human hyaluronan synthase. Biochem Biophys Res Commun 222:816-20View Article
  Jiang D, Liang J, Fan J, Yu S, Chen S, Luo Y, Prestwich GD, Mascarenhas MM, Garg HG, Quinn DA, Homer RJ, Goldstein DR, Bucala R, Lee PJ, Medzhitov R, Noble PW (2005) Regulation of lung injury and repair by Toll-like receptors and hyaluronan. Nat Med 11:1173-179View Article
  Kanda N, Watanabe S (2005) Regulatory roles of sex hormones in cutaneous biology and immunology. J Dermatol Sci 38:1-View Article
  Kondo T, Chichibu K, Usui H, Matsuura T, Shichijo S, Yokoyama M (1991) Rapid assay of hyaluronic acid in serum. Jpn J Clin Pathol 39:536-40
  Longas MO, Russell CS, He XY (1987) Evidence for structural changes in dermatan sulfate and hyaluronic acid with aging. Carbohydr Res 159:127-36View Article
  Manuskiatti W, Maibach HI (1996) Hyaluronic acid and skin: wound healing and aging. Int J Dermatol 35:539-44View Article
  Margelin D, Medaisko C, Lombard D, Picard J, Fourtanier A (1996) Hyaluronic acid and dermatan sulfate are selectively stimulated by retinoic acid in irradiated and nonirradiated hairless mouse skin. J Invest Dermatol 106:505-09View Article
  Postlethwaite AE, Smith GN Jr, Lachman LB, Endres RO, Poppleton HM, Hasty KA, Seyer JM, Kang AH (1989) Stimulation of glycosaminoglycan synthesis in cultured human dermal fibroblasts by interleukin 1: induction of hyaluronic acid synthesis by natural and recombinant interleukin 1?s and synthetic interleukin 1 beta peptide 163-171. J Clin Invest 83:629-36View Article
  R?ck K, Grandoch M, Majora M, Krutmann J, Fischer JW (2011) Collagen fragments inhibit hyaluronan synthesis in skin fibroblasts in response to ultraviolet B (UVB): new insights into mechanisms of matrix remodeling. J Biol Chem 286:18268-8276View Article
  Simpson RM, Meran S, Thomas D, Stephens P, Bowen T, Steadman R, Phillips A (2009) Age-related changes in pericellular hyaluronan organization leads to impaired dermal fibroblast to myofibroblast differentiation. Am J Pathol 175:1915-928View Article
  Stern R (2004) Hyaluronan catabolism: a new metabolic pathway. Eur J Cell Biol 83:317-25View Article
  Südel KM, Venzke K, Mielke H, Breitenbach U, Mundt C, Jaspers S, Koop U, Sauermann K, Knussman-Hartig E, Moll I, Gercken G, Young AR, St?b F, Wenck H, Gallinat S (2005) Novel aspects of intrinsic and extrinsic aging of human skin: beneficial effects of soy extract. Photochem Photobiol 81:581-87View Article
  Suganuma K, Nakajima H, Otsuki M, Imokawa G (2010) Astaxanthin attenuates the UVA-induced-regulation of matrix-metalloproteinase-1 and skin fibroblast elastase in human dermal fibroblasts. J Dermatol Sci 58:1361-362View Article
  Sugiyama Y, Shimada A, Sayo T, Sakai S, Inoue S (1998) Putative hyaluronan synthase mRNA are expressed in mouse skin and TGF-beta upregulates their expression in cultured human skin cells. J Invest Dermatol 110:116-21View Article
  Suzuki M, Asplund T, Yamashita H, Heldin CH, Heldin P (1995) Stimulation of hyaluronan biosynthesis by platelet-derived growth factor-BB and tr
• 作者单位：Shuko Terazawa (1)
  Hiroaki Nakajima (2)
  Keisuke Tobita (2)
  Genji Imokawa (1)
  
  1. Research Institute for Biological Functions, Chubu University, 1200 Matsumoto, Kasugai, Aichi, 487-8501, Japan
  2. School of Bioscience and Biotechnology, Tokyo University of Technology, Tokyo, Japan
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biomedicine
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-0778

文摘

Although it has been reported that levels of hyaluronan are decreased in the dermis of aged skin, little is known about the cellular mechanism(s) underlying that hyaluronan deficiency. Since hyaluronan is produced by dermal fibroblasts and is secreted into the surrounding dermal tissues, we examined the secretion of hyaluronan by dermal fibroblasts and characterized its cellular mechanism using real-time RT-PCR and western blotting for its synthesizing and degrading enzymes, hyaluronan synthase and hyaluronidase, respectively. The secretion of hyaluronan by dermal fibroblasts derived from differently aged human donors, was higher in the younger human fibroblasts tested (0 and 19?years old) compared to the older human fibroblasts tested (39, 56 and 77?years old). The relative secretion levels of hyaluronan by the different human fibroblasts tested were attributable to the relative expression of hyaluronan synthases 1, 2, 3 but not hyaluronidases 1, 2 enzymes at the gene and protein levels among those fibroblasts. These findings indicate that the deficiency of hyaluronan in the aged dermis might result from the down-regulation in the potential of older human fibroblasts to secrete hyaluronan and that decrease in secretory potential is mainly associated with the down-regulated expression of hyaluronan synthases, especially hyaluronan synthase 2, but not with the expression levels of hyaluronidases.