Distinct intraspecific variations of garlic (Allium sativum L.) revealed by the exon–intron sequences of the alliinase gene
详细信息 查看全文
- 作者：Aki Endo (1)
Yukiko Imai (1)
Mizuho Nakamura (1)
Eri Yanagisawa (1)
Takaaki Taguchi (1)
Kosuke Torii (2)
Hidenobu Okumura (2)
Koji Ichinose (1) - 关键词：Alliinase ; Intron ; Allium sativum ; Intraspecific variation
- 刊名：Journal of Natural Medicines
- 出版年：2014
- 出版时间：April 2014
- 年：2014
- 卷：68
- 期：2
- 页码：442-447
- 全文大小：442 KB
- 参考文献：1. Rivlin RS (2001) Historic perspective on the use of garlic. J Nutr 131:951S-54S
2. Saito H (ed) (2000) Garlic science. Asakura Publishing Company, Tokyo (in Japanese)
3. Nishiyama N, Moriguchi T, Saito H (1997) Beneficial effects of aged garlic extract on learning and memory impairment in the senescence-accelerated mouse. Exp Gerontol 32:149-60 CrossRef
4. Nishiyama N, Moriguchi T, Morihara N, Saito H (2001) Ameliorative effect of / S-allylcysteine, a major thioallyl constituent in aged garlic extract, on learning deficits in senescence-accelerated mice. J Nutr 131:1093S-095S
5. Matsumoto T, Stark TD, Hofman T (2013) In vitro activity-guided identification of antioxidants in aged garlic extract. J Agric Food Chem 61:3059-067 CrossRef
6. Allisona GL, Loweb GM, Rahmanb K (2012) Aged garlic extract inhibits platelet activation by increasing intracellular cAMP and reducing the interaction of GPIIb/IIIa receptor with fibrinogen. Life Sci 91:1275-280 CrossRef
7. Ried K, Frank OR, Stocks NP (2013) Aged garlic extract reduces blood pressure in hypertensives: a dose-response trial. Eur J Clin Nutr 67:64-0 CrossRef
8. Jones MG, Hughes J, Tregova A, Milne J, Tomsett B, Collin HA (2004) Biosynthesis of the flavor precursors of onion and garlic. J Exp Bot 55:1903-918 CrossRef
9. Amagase H (2006) Clarifying the real bioactive constituents of garlic. J Nutr 136:716S-25S
10. Sang EB, Cho SY, Won YD, Lee SH, Park HJ (2012) A comparative study of the different analytical methods for analysis of / S-allyl cysteine in black garlic by HPLC. LWT Food Sci Technol 46:532-35 CrossRef
11. Maa? HI, Klaas M (1995) Infraspecific differentiation of garlic ( / Allium sativum L.) by isozyme and RAPD markers. Theor Appl Genet 91:89-7 CrossRef
12. Al-Zahim M, Newbury HJ, Ford-Loyd BV (1997) Classification of genetic variation in garlic ( / Allium sativum L.) revealed by RAPD. HortScience 32:1102-104
13. Ohri D, Pistrik K (2001) Phenology and genome size variation in / Allium L.—a tight correlation? Plant Biol 3:650-54 CrossRef
14. King JJ, Bradeen JM, Bark O, McCallum JA, Havey MJ (1998) A low-density genetic map of onion reveals a role for tandem duplication in the evolution of an extremely large diploid genome. Theor Appl Genet 96:52-2 CrossRef
15. Hong C-J, Etoh T (1996) Fertile clones from garlic ( / Allium sativum L.) abundant around the Tien Shan Mountains. Breed Sci 46:349-53
16. Van Dijk P (1993) Survey and characterization of potyviruses and their strains of Allium species. Neth J Plant Pathol 99(Suppl. 2):1-8 CrossRef
17. Chen J, Chen J, Adams MJ (2001) Molecular characterization of a complex mixture of viruses in garlic with mosaic symptoms in China. Arch Virol 146:1841-853 CrossRef
18. Takaki F, Sano T, Yamashita K, Fujita T, Ueda K, Kato T (2005) Complete nucleotide sequences of attenuated and severe isolates of Leek yellow stripe virus from garlic in northern Japan: identification of three distinct virus types in garlic and leek world-wide. Arch Virol 150:1135-149 CrossRef
19. Yoshida N, Shimura H, Yamashita K, Suzuki M, Masuta C (2012) Variability in the P1 gene helps to refine phylogenetic relationships among leek stripe virus isolates from garlic. Arch Virol 157:147-53 CrossRef
20. den Dunnen JT, Antonarakis SE (2002) Mutation nomenclature extensions and suggestions to describe complex mutations: a discussion. Hum Mutat 15:7-2 CrossRef - 作者单位：Aki Endo (1)
Yukiko Imai (1)
Mizuho Nakamura (1)
Eri Yanagisawa (1)
Takaaki Taguchi (1)
Kosuke Torii (2)
Hidenobu Okumura (2)
Koji Ichinose (1)
1. Research Institute of Pharmaceutical Sciences, Musashino University, 1-1-20 Shinmachi, Nishitokyo, Tokyo, 202-8585, Japan
2. Noevir Co., Ltd, 112-1 Okada-cho, Higashiomi, Shiga, 527-8588, Japan - ISSN：1861-0293
文摘
Garlic (Allium sativum L.) has been used worldwide as a food and for medicinal purposes since early times. Garlic cultivars exhibit considerable morphological diversity despite the fact that they are mostly sterile and are grown only by vegetative propagation of cloves. Considerable recombination occurs in garlic genomes, including the genes involved in secondary metabolites. We examined the genomic DNAs (gDNAs) from garlic, encoding alliinase, a key enzyme involved in organosulfur metabolism in Allium plants. The 1.7-kb gDNA fragments, covering three exons (2, 3, and 4) and all four introns, were amplified from total DNAs prepared from garlic samples produced in Asia and Europe, leading to 73 sequences in total: Japan (JPN), China (CHN), India (IND), Spain (ESP), and France (FRA). The exon sequences were highly conserved among all the sequences, probably reflecting the fully functional alliinase associated with the flavor quality. Distinct intraspecific variations were detected for all four intron sequences, leading to the haplotype classifications. A close relationship between JPN and CHN was observed for all four introns, whereas IND showed a more divergent distribution. ESP and FRA afforded clearly different variants compared with those from Asian sequences. The present study provides information that could be useful in the development of an additional molecular marker for garlic authentication and quality control.