In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsutum L.)
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- 作者：D. C. Agrawal ; A. K. Banerjee ; R. R. Kolala ; A. B. Dhage ; A. V. Kulkarni ; S. M. Nalawade ; S. Hazra and K. V. Krishnamurthy
- 关键词：Cotton ; Gossypium hirsutum L. ; Micropropagation
- 刊名：Plant Cell Reports
- 出版年：1997
- 出版时间：June 1997
- 年：1997
- 卷：16
- 期：9
- 页码：647-652
- 全文大小：312 KB
文摘
Induction of multiple shoots in cotton (Gossypium hirsutum L. cv. Anjali-LRK 516) has been achieved with cotyledonary nodes devoid of cotyledons and apical meristems. Explants from 35-day-old seedlings yielded the maximum number of shoots (4.7 shoots/explant) using Murashige and Skoog (MS) basal medium supplemented with 6-benzylaminopurine and kinetin (2.5 mg/1 each). Explants from 35-day-old seedlings raised in glass bottles produced a higher number of multiple shoots (8.3 shoots/explant) than those grown in glass tubes and cultured on the same shoot induction medium. Elongation of multiple shoots was obtained on liquid or agar MS basal medium without phytohormones. In vitro shoots were rooted on half-strength agar-solidified MS basal medium or with 0.05 or 0.1 mg/1 naphthaleneacetic acid. Hardening and survival of tissue culture plantlets was 95 % under greenhouse conditions.