miRNAome analysis associated with anatomic and transcriptomic investigations reveal the polar exhibition of corky split vein in boron deficient Citrus sinensis

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• 作者：Chengquan Yang ; Tao Liu ; Fuxi Bai ; Nannan Wang…
• 关键词：Corky split vein ; Anatomy ; miRNAome ; Lignification ; Cell division ; Orderless stage transition
• 刊名：Molecular Genetics and Genomics
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：290
• 期：5
• 页码：1639-1657
• 全文大小：1,902 KB
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• 作者单位：Chengquan Yang (1) (2) (3)
  Tao Liu (1) (2) (3)
  Fuxi Bai (1) (2) (3)
  Nannan Wang (1) (2) (3)
  Zhiyong Pan (1) (2) (3)
  Xiang Yan (1) (2) (3)
  ShuAng Peng (1) (2) (3)
  
  1. College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China
  2. Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan, 430070, China
  3. Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, Wuhan, 430070, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biochemistry
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1617-4623

文摘

Corky split vein can develop under long-term boron deficient conditions in Citrus sinensis L. Osbeck cv. Newhall. This symptom only occurs in the upper rather than the lower epidermis of old leaves. Our previous study demonstrated that vascular hypertrophy was involved in the symptoms, and the 3rd developmental stage of corky split vein (BD3) was the critical stage for phenotype formation. Here, we performed an intensive study on the BD3 vein and its control sample (CK3 vein). A lignin test demonstrated that the lignin content in BD3 vein was approximately 1.7 times more than the CK3 vein. Anatomical investigation of the corky split vein indicated that the upper epidermis was destroyed by overgrowing vascular cells, and the increased lignin may contribute to vascular cell differentiation and wounding-induced lignification. In a subsequent small RNA sequencing of the BD3 and CK3 veins, 99 known miRNAs and 22 novel miRNAs were identified. Comparative profiling of these miRNAs demonstrated that the 57 known miRNAs and all novel miRNAs exhibited significant expression differences between the two small RNAs libraries of the BD3 and CK3 veins. Associated with our corresponding digital gene expression data, we propose that the decreased expression of two miRNAs, csi-miR156b and csi-miR164, which leads to the up-regulation of their target genes, SPLs (csi-miR156b-targeted) and CUC2 (csi-miR164-targeted), may promote vascular cell division and orderless stage transition in old leaves. Keywords Corky split vein Anatomy miRNAome Lignification Cell division Orderless stage transition