Amino acids influence the glucose uptake through GLUT4 in CHO-K1 cells under high glucose conditions

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• 作者：Radhakrishnan Selvi ; Narayanasamy Angayarkanni ; Begum Asma ; Thiagarajan Seethalakshmi and Srinivasan Vidhya
• 关键词：Amino acids ; Glucose uptake ; GLUT4
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2010
• 出版时间：November 2010
• 年：2010
• 卷：344
• 期：1-2
• 页码：43-53
• 全文大小：752.0 KB

文摘

According to studies earlier, amino acids have proven to be antidiabetic, antiglycating, and anticataractogenic. The present study was to explore whether amino acids as mixtures could enhance glucose uptake in CHO-K1 cells specifically. The cells in F-12K1 serum-free medium were exposed to normal (7 mM) and high glucose (12, 17 and 27 mM) in the presence and absence of amino acids mixture (AAM) in varying concentration (2.5, 5 and 10 mM). The mixture 5 and 10 mM AAM increased the 2-deoxyglucose (2DG) uptake at all glucose concentration significantly. There was also a significant increase in the GLUT4 (glucose transporter) translocation as revealed by flow cytometer. Addition of a mixture of amino acids was found to improve cell viability, which got altered by high glucose in the CHO-K1 cells. Amino acids as mixture had a beneficial effect in improving the net utilization of glucose as an additive effect with insulin.