Fluorescent molecularly imprinted polymer based on Navicula sp. frustules for optical detection of lysozyme

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• 作者：Guat Wei Lim ; Jit Kang Lim ; Abdul Latif Ahmad…
• 关键词：Fluorescent ; Molecularly imprinted polymer ; Navicula sp. frustules ; FITC ; Lysozyme recognition
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：408
• 期：8
• 页码：2083-2093
• 全文大小：2,048 KB
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• 作者单位：Guat Wei Lim (1)
  Jit Kang Lim (1) (2)
  Abdul Latif Ahmad (1)
  Derek Juinn Chieh Chan (1)
  
  1. School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, Seri Ampangan, 14300, Nibong Tebal, Seberang Perai Selatan, Pulau Pinang, Malaysia
  2. Department of Physics, Carnegie Mellon University, Pittsburgh, PA, 15213, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

The direct correlation between disease and lysozyme (LYZ) levels in human body fluids makes the sensitive and convenient detection of LYZ the focus of scientific research. Fluorescent molecularly imprinted polymer has emerged as a new alternative for LYZ detection in order to resolve the limitation of immunoassays, which are expensive, unstable, require complex preparation, and are time consuming. In this study, a novel fluorescence molecularly imprinted polymer based on Navicula sp. frustules (FITC-MIP) has been synthesized via post-imprinting treatment for LYZ detection. Navicula sp. frustules were used as supported material because of their unique properties of moderate surface area, reproducibility, and biocompatibility, to address the drawbacks of nanoparticle core material with low adsorption capacity. The FITC acts as recognition signal and optical readout, whereas MIP provides LYZ selectivity. The synthesized FITC-MIP showed a response time as short as 5 min depending on the concentration of LYZ. It is found that the LYZ template can significantly quench the fluorescence intensity of FITC-MIP linearly within a concentration range of 0 to 0.025 mg mL–1, which is well described by Stern-Volmer equation. The FITC-MIP can selectively and sensitively detect down to 0.0015 mg mL–1 of LYZ concentration. The excellent sensing performance of FITC-MIP suggests that FITC-MIP is a potential biosensor in clinical diagnosis applications.