Properties of hybridization isotherms upon binding of ligands on microchips

详细信息    查看全文

• 作者：M. V. Golovkin (1)
  O. V. Matveeva (1)
  Yu. D. Nechipurenko (1) (2)
  
• 关键词：microchip ; hybridization ; mathematical modeling ; thermodynamics ; Langmuir isotherm ; adsorption
• 刊名：Biophysics
• 出版年：2009
• 出版时间：October 2009
• 年：2009
• 卷：54
• 期：5
• 页码：581-583
• 全文大小：160KB
• 参考文献：1. Yu. D. Nechipurenko, Ross. Khim. Zh. 51, 23 (2007).
  2. C. J. Burden, Y. Pittelkow, and S. R. Wilson, J. Phys. Condens. Matter 18, 5545 (2006). dx.doi.org/10.1088/0953-8984/18/23/024">CrossRef
  3. Y. Zhang, D. A. Hammer, and D. J. Graves, Biophys. J. 89, 2950 (2005). dx.doi.org/10.1529/biophysj.104.058552">CrossRef
  4. J. Hooyberghs, P. Van Hummelen, and E. Carlon, Nucl. Acids Res. 37, e53 (2009). dx.doi.org/10.1093/nar/gkp109">CrossRef
  
• 作者单位：M. V. Golovkin (1)
  O. V. Matveeva (1)
  Yu. D. Nechipurenko (1) (2)
  
  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia
  2. Physical Faculty, Moscow State University, Moscow, 119992, Russia
  
• ISSN：1555-6654

文摘

A simple thermodynamic model describing microarray oligo-target hybridization has been constructed. The relationship between the hybridization signal intensity and Gibbs free energy for oligo-target duplex formation has been considered. The behavior of this function, which we called energy hybridization isotherm, in response to target concentration change was modeled at different ratios of oligo/target concentrations. The results of modeling were compared with the relevant and currently available data from microarray adsorption experiments.