Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for detection of beet necrotic yellow vein virus
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- 作者：Mohammad Amin Almasi ; Galavizh Almasi
- 关键词：Beet necrotic yellow vein virus (BNYVV) ; DAS ; ELISA ; IC ; RT ; LAMP ; RT ; LAMP ; Sugar beet
- 刊名：Archives of Virology
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：162
- 期：2
- 页码：495-500
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Virology; Medical Microbiology; Infectious Diseases;
- 出版者：Springer Vienna
- ISSN：1432-8798
- 卷排序：162
文摘
Sugar beet can be infected by many different viruses that can reduce yield; beet necrotic yellow vein virus (BNYVV) is one of the most economically important viruses of this crop plant. This report describes a new reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for identification of BNYVV. In addition, a novel immunocapture (IC) RT-LAMP assay for rapid and easy detection (without RNA extraction) of BNYVV was developed here and compared with DAS-ELISA and RT-LAMP assays. Our results show that the IC-RT-LAMP assay is a highly reliable alternative assay for identification of BNYVV.