Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae

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• 作者：Filippe Elias de Freitas Soares ; José Humberto de Queiroz…
• 关键词：Nematophagous fungi ; Angiostrongylus vasorum ; Protease
• 刊名：BMC Research Notes
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：7
• 期：1
• 全文大小：339 KB
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  7. Soares, FEF, Braga, FR, Araújo, JV, Mozer, LR, Lima, WS, Queiroz, JH (2013) Nematicidal activity of three novel extracellular proteases of the nematophagous fungus Monacrosporium sinense. Parasitol Res 112: pp. 1557-1565 CrossRef
  8. Soares, FEF, Braga, FR, Araújo, JV, Lima, WS, Mozzer, LR, Queiroz, JH (2013) Optimization of protease production by fungus Monacrosporium thaumasium and its action against Angiostrongylus vasorum larvae. Rev Bras Parasitol Vet 22: pp. 285-288 CrossRef
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  12. Braga, FR, Carvalho, RO, Araujo, JM, Silva, AR, Araujo, JV, Lima, WS, Tavela, AO, Ferreira, SR (2009) Predatory activity of the fungi Duddingtonia flagrans, Monacrosporium thaumasium, Arthrobotrys sinensis and Arthrobotrys robusta on Angiostrongylus vasorum first stage larvae. J Helminthol 83: pp. 303-308 CrossRef
  13. Braga, FR, Araujo, JM, Silva, AR, Araújo, JV, Carvalho, RO, Soares, FEF, Queiroz, JH, Geniêr, HLA (2011) Ovicidal action of a crude enzymatic extract of fungus Pochonia chlamydosporia against Ancylostoma sp. eggs. Rev Soc Bras Med Trop 44: pp. 116-118 CrossRef
  
• 刊物主题：Biomedicine general; Medicine/Public Health, general; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1756-0500

文摘

Background The predatory nematophagous fungus Arthrobotrys sinensis (SF53) produces three proteases with nematicidal activity when grown on solid media culture. However, the proteolytic profile produced by this fungus, when grown in liquid culture medium remains unknown. Findings Thus, the objective of this work was to evaluate the production of proteases from nematophagous fungus Arthrobotrys sinensis in liquid medium and its nematicidal activity on first stage larvae of A. vasorum. Proteases were obtained in its crude form, using Whatman no.1 filter paper, followed by centrifugation for 5?min at 10?×?g and 4°C. A zymogram was performed with co-polymerized casein in an acrylamide gel as substrate. An in vitro assay to evaluate the nematicidal action of the proteases of A. sinensis (SF53) produced in liquid medium on A. vasorum L1 was conducted. By the analysis of the zymogram, it was observed a single halo at the beginning of digestion of the gel, suggesting that the three proteases of SF53 are produced in an enzymatic complex of large molecular weight. Regarding nematicidal activity, within 24?hours, the proteases produced in liquid medium of A. sinensis (SF53) showed a percentage reduction of 64% on the number of L1 of A. vasorum. Conclusion In the present work, it is suggested that the three proteases of SF53 are produced in an enzymatic complex and was also demonstrated that these enzymes were effective in destroying A. vasorum L1.