Functiogenesis of the embryonic central nervous system revealed by optical recording with a voltage-sensitive dye

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• 作者：Katsushige Sato ; Yoko Momose-Sato
• 关键词：Optical recording ; Voltage ; sensitive dye ; Chick embryo ; Development ; Olfactory system ; Oscillation
• 刊名：The Journal of Physiological Sciences
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：67
• 期：1
• 页码：107-119
• 全文大小：
• 刊物主题：Human Physiology; Neurosciences; Animal Biochemistry; Animal Physiology; Cell Physiology; Neurobiology;
• 出版者：Springer Japan
• ISSN：1880-6562
• 卷排序：67

文摘

Clarification of the functiogenesis of the embryonic central nervous system (CNS) has long been problematic, because conventional electrophysiological techniques have several limitations. First, early embryonic neurons are small and fragile, and the application of microelectrodes is challenging. Second, the simultaneous monitoring of electrical activity from multiple sites is limited, and as a consequence, spatiotemporal response patterns of neural networks cannot be assessed. We have applied multiple-site optical recording with a voltage-sensitive dye to the embryonic CNS and paved a new way to analyze the functiogenesis of the CNS. In this review, we discuss key points of optical recording in the embryonic CNS and introduce recent progress in optical investigations on the embryonic CNS with special emphasis on the development of the chick olfactory system. The studies clearly demonstrate the usefulness of voltage-sensitive dye recording as a powerful tool for elucidating the functional organization of the vertebrate embryonic CNS.