Lead Intoxication Synergies of the Ethanol-Induced Toxic Responses in Neuronal Cells—PC12

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• 作者：V. Kumar ; V. K. Tripathi ; S. Jahan ; M. Agrawal ; A. Pandey…
• 关键词：Neurotoxicity ; Pb ; Ethanol ; PC12 cells
• 刊名：Molecular Neurobiology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：52
• 期：3
• 页码：1504-1520
• 全文大小：2,411 KB
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• 作者单位：V. Kumar (1) (2)
  V. K. Tripathi (1) (2)
  S. Jahan (1) (2)
  M. Agrawal (1) (2)
  A. Pandey (1) (2)
  V. K. Khanna (1) (2)
  A. B. Pant (1) (2)
  
  1. In Vitro Toxicology Laboratory, Indian Institute of Toxicology Research, P.O. Box: 80, MG Marg, Lucknow, 226001, UP, India
  2. Council of Scientific & Industrial Research, New Delhi, India
  
• 刊物主题：Neurosciences; Neurobiology; Cell Biology; Neurology;
• 出版者：Springer US
• ISSN：1559-1182

文摘

Lead (Pb)-induced neurodegeneration and its link with widespread neurobehavioral changes are well documented. Experimental evidences suggest that ethanol could enhance the absorption of metals in the body, and alcohol consumption may increase the susceptibility to metal intoxication in the brain. However, the underlying mechanism of ethanol action in affecting metal toxicity in brain cells is poorly understood. Thus, an attempt was made to investigate the modulatory effect of ethanol on Pb intoxication in PC12 cells, a rat pheochromocytoma. Cells were co-exposed to biological safe doses of Pb (10 μM) and ethanol (200 mM), and data were compared to the response of cells which received independent exposure to these chemicals at similar doses. Ethanol (200 mM) exposure significantly aggravated the Pb-induced alterations in the end points associated with oxidative stress and apoptosis. The finding confirms the involvement of reactive oxygen species (ROS)-mediated oxidative stress, and impairment of mitochondrial membrane potential, which subsequently facilitate the translocation of triggering proteins between cytoplasm and mitochondria. We further confirmed the apoptotic changes due to induction of mitochondria-mediated caspase cascade. These cellular changes were found to recover significantly, if the cells are exposed to N-acetyl cysteine (NAC), a known antioxidant. Our data suggest that ethanol may potentiate Pb-induced cellular damage in brain cells, but such damaging effects could be recovered by inhibition of ROS generation. These results open up further possibilities for the design of new therapeutics based on antioxidants to prevent neurodegeneration and associated health problems. Keywords Neurotoxicity Pb Ethanol PC12 cells