Activated platelet-rich plasma improves adipose-derived stem cell transplantation efficiency in injured articular cartilage

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• 作者：Phuc Van Pham (1)
  Khanh Hong-Thien Bui (2)
  Dat Quoc Ngo (3)
  Ngoc Bich Vu (1)
  Nhung Hai Truong (1)
  Nhan Lu-Chinh Phan (1)
  Dung Minh Le (1)
  Triet Dinh Duong (2)
  Thanh Duc Nguyen (2)
  Vien Tuong Le (2)
  Ngoc Kim Phan (1)
  
• 关键词：Adipose tissue ; derived stem cells ; Articular cartilage injury ; Joint failure ; Mesenchymal stem cells ; Osteoarthritis ; Platelet ; rich plasma
• 刊名：Stem Cell Research & Therapy
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：4
• 期：4
• 全文大小：
• 作者单位：Phuc Van Pham (1)
  Khanh Hong-Thien Bui (2)
  Dat Quoc Ngo (3)
  Ngoc Bich Vu (1)
  Nhung Hai Truong (1)
  Nhan Lu-Chinh Phan (1)
  Dung Minh Le (1)
  Triet Dinh Duong (2)
  Thanh Duc Nguyen (2)
  Vien Tuong Le (2)
  Ngoc Kim Phan (1)
  
  1. Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University, 227 Nguyen Van Cu, District 5, Ho Chi Minh City, Vietnam
  2. University of Medical Center, Ho Chi Minh University of Medicine and Pharmacy, 215 Hong Bang, District 5, Ho Chi Minh City, Vietnam
  3. Department of Pathology, University of Medicine and Pharmacy, 217 Hong Bang, District 5, Ho Chi Minh City, Vietnam
  
• ISSN：1757-6512

文摘

Introduction Adipose-derived stem cells (ADSCs) have been isolated, expanded, and applied in the treatment of many diseases. ADSCs have also been used to treat injured articular cartilage. However, there is controversy regarding the treatment efficiency. We considered that ADSC transplantation with activated platelet-rich plasma (PRP) may improve injured articular cartilage compared with that of ADSC transplantation alone. In this study, we determined the role of PRP in ADSC transplantation to improve the treatment efficiency. Methods ADSCs were isolated and expanded from human adipose tissue. PRP was collected and activated from human peripheral blood. The effects of PRP were evaluated in vitro and in ADSC transplantation in vivo. In vitro, the effects of PRP on ADSC proliferation, differentiation into chondrogenic cells, and inhibition of angiogenic factors were investigated at three concentrations of PRP (10%, 15% and 20%). In vivo, ADSCs pretreated with or without PRP were transplanted into murine models of injured articular cartilage. Results PRP promoted ADSC proliferation and differentiation into chondrogenic cells that strongly expressed collagen II, Sox9 and aggrecan. Moreover, PRP inhibited expression of the angiogenic factor vascular endothelial growth factor. As a result, PRP-pretreated ADSCs improved healing of injured articular cartilage in murine models compared with that of untreated ADSCs. Conclusion Pretreatment of ADSCs with PRP is a simple method to efficiently apply ADSCs in cartilage regeneration. This study provides an important step toward the use of autologous ADSCs in the treatment of injured articular cartilage.