Long-term copper (Cu2+) exposure impacts on auxin, nitric oxide (NO) metabolism and morphology of Arabidopsisthaliana L.

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• 作者：Zsuzsanna Kolbert (1) kolzsu@bio.u-szeged.hu
  Andrea Pet? (1)
  Nóra Lehotai (1)
  Gábor Feigl (1)
  László Erdei (1)
• 关键词：Auxin – Copper – Nitric oxide – Plant morphology
• 刊名：Plant Growth Regulation
• 出版年：2012
• 出版时间：November 2012
• 年：2012
• 卷：68
• 期：2
• 页码：151-159
• 全文大小：2.6 MB
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• 作者单位：1. Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary
• ISSN：1573-5087

文摘

Plants are able to dynamically adapt to their environment by reprogramming of their growth and development. Copper (Cu2+) excess modifies shoot and root architecture of plants by a lesser known mechanism, therefore the involvement of a major hormone component (auxin) and a signal molecule (nitric oxide) in Cu2+-induced morphological responses were studied in Arabidopsis using microscopic methods. Auxin-inducible gene expression was visualized in DR5::GUS Arabidopsis and nitric oxide (NO) levels were detected by DAF-FM fluorophore in the stem and root system. Copper excess caused the inhibition of stem and root growth of Arabidopsis, during which cell elongation, division and expansion were also affected. The symptoms of stress-induced morphogenic response were found in the root system of 25 μM Cu2+-treated plants. In both organs, the decrease of auxin-dependent gene expression was found, which can partly explain the growth inhibitions. Besides hormonal system, nitric oxide metabolism was also affected by Cu2+. In root tips, copper excess induced NO generation, while NO content in lateral roots was not affected by the treatments. Using nia1nia2 mutants, nitrate reductase enzyme as a putative source of Cu2+-induced NO was identified in Arabidopsis primary roots.