Benzothiazole bipyridine complexes of ruthenium(II) with cytotoxic activity
详细信息 查看全文
- 作者：Caitriona B. Spillane ; Nicholas C. Fletcher ; Sandra M. Rountree ; Hendrik van den Berg ; Severine Chanduloy ; Joy L. Morgan and F. Richard Keene
- 关键词：Ruthenium ; 2 ; 2′ ; Bipyridine ; Cytotoxicity ; Benzothiazol ; DNA binding
- 刊名：Journal of Biological Inorganic Chemistry
- 出版年：2007
- 出版时间：August, 2007
- 年：2007
- 卷：12
- 期：6
- 页码：797-807
- 全文大小：404 KB
文摘
A series of benzothiazole-substituted trisbipyridine ruthenium(II) analogues {[Ru(bpy)2(4,5′-bbtb)]2+, [Ru(bpy)2(5,5′-bbtb)]2+ and [Ru(bpy)2(5-mbtb)]2+ [bpy is 2,2′-bipyridine, bbtb is bis(benzothiazol-2-yl)-2,2′-bipyridine, 5-mbtb is 5-(benzothiazol-2-yl),5′-methyl-2,2′-bipyridine]} have been prepared and compared with the complex [Ru(bpy)2(4,4′-bbtb)]2+ reported previously. From the UV–vis spectral studies, substitution at the 5-position of the bpy causes the ligand-centred transitions to occur at considerably lower energy than for those with the functionality at the 4-position, while at the same time causing the emission to be effectively quenched. However, substitution at the 4-position causes the metal-to-ligand charge transfer to occur at lower energies. Fluorescent intercalator displacement studies indicate that the doubly substituted complexes displace ethidium bromide from a range of oligonucleotides, with the greater preference shown for bulge and hairpin sequences by the Λ enantiomer. Since the complexes only show small variation in the UV–vis spectra on the introduction of calf thymus DNA and a small increase in fluorescence they do not appear to be intercalators, but appear to associate within one of the grooves. All of the reported bisbenzothiazole complexes show reasonable cytotoxicity against a range of human cancer cell lines.