Detection of m class="a-plus-plus">Strongylus vulgarism> in equine faecal samples by real-time PCR and larval culture – method comparison and occurrence assessment

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• 作者：A. Kaspar ; K. Pfister ; M. K. Nielsen ; C. Silaghi ; H. Fink…
• 关键词：Strongylus vulgaris ; Strongyle ; Equine ; Larval culture ; Real ; time PCR ; Germany
• 刊名：BMC Veterinary Research
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：13
• 期：1
• 全文大小：605KB
• 刊物主题：Veterinary Medicine/Veterinary Science; Zoology; Transgenics;
• 出版者：BioMed Central
• ISSN：1746-6148
• 卷排序：13

文摘

BackgroundStrongylus vulgaris has become a rare parasite in Germany during the past 50 years due to the practice of frequent prophylactic anthelmintic therapy. To date, the emerging development of resistance in Cyathostominae and Parascaris spp. to numerous equine anthelmintics has changed deworming management and the frequency of anthelmintic usage. In this regard, reliable detection of parasitic infections, especially of the highly pathogenic S. vulgaris is essential. In the current study, two diagnostic methods for the detection of infections with S. vulgaris were compared and information on the occurrence of this parasite in German horses was gained. For this purpose, faecal samples of 501 horses were screened for S. vulgaris with real-time PCR and an additional larval culture was performed in samples of 278 horses. A subset of 26 horses underwent multiple follow-up examinations with both methods in order to evaluate both the persistence of S. vulgaris infections and the reproducibility of each diagnostic method.