Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichiacoli
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- 作者：Yoshitake Orikasa ; Takanori Nishida ; Akiko Yamada ; Reiko Yu ; Kazuo Watanabe ; Akira Hase ; Naoki Morita and Hidetoshi Okuyama
- 关键词：DHA biosynthesis genes ; Docosahexaenoic acid ; Eicosapentaenoic acid ; Moritella marina strain MP ; 1 ; Polyunsaturated fatty acids
- 刊名：Biotechnology Letters
- 出版年：2006
- 出版时间：November, 2006
- 年：2006
- 卷：28
- 期：22
- 页码：1841-1847
- 全文大小：211 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbiology
Biotechnology
Applied Microbiology
Biochemistry - 出版者：Springer Netherlands
- ISSN：1573-6776
文摘
The docosahexaenoic acid (DHA) biosynthesis gene cluster (pDHA3) from the DHA-producing Moritella marina strain MP-1 includes the genes pfaA, pfaB, pfaC, and pfaD, which are similar to the genes of polyketide biosynthesis. When this cluster was co-expressed in Escherichia coli with M. marina MP-1 pfaE, which encodes phosphopantetheinyl transferase, DHA was biosynthesized. The maximum production of DHA (5% of total fatty acids) was observed at 15°C. This is the first report of the recombinant production of DHA in a polyketide biosynthesis mode.