Silica Colloidal Crystals as Emerging Materials for High-Throughput Protein Electrophoresis

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• 作者：Nadine K. Njoya (1)
  Robert E. Birdsall (1)
  Mary J. Wirth (1)
  
• 关键词：colloidal crystal ; electrophoresis ; high throughput ; miniaturization ; monoclonal antibody ; plate height
• 刊名：The AAPS Journal
• 出版年：2013
• 出版时间：October 2013
• 年：2013
• 卷：15
• 期：4
• 页码：962-969
• 全文大小：
• 作者单位：Nadine K. Njoya (1)
  Robert E. Birdsall (1)
  Mary J. Wirth (1)
  
  1. Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana, 49707, USA
  
• ISSN：1550-7416

文摘

Silica colloidal crystals are a new type of media for protein electrophoresis, and they are assessed for their promise in rapidly measuring aggregation of monoclonal antibodies. The nature of silica colloidal crystals is described in the context of the need for a high-throughput separation tool for optimizing the formulations of protein drugs for minimal aggregation. The fundamental relations between molecular weight and mobility in electrophoresis are used to make a theoretical comparison of selectivity between gels and colloidal crystals. The results show that the selectivity is similar for these media, but slightly higher, 10%, for gels, and the velocity is inherently lower than that for gels due to the smaller free volume fraction. These factors are more than compensated for by lower broadening in colloidal crystals. These new media give plate heights of only 0.15 for the antibody monomer and 0.42 for the antibody dimer. The monoclonal antibody is separated from its dimer in 72s over a distance of only 6.5mm. This is five times faster than size-exclusion chromatography, with more than tenfold miniaturization, and amenable to parallel separations, all of which are promising for the design of high-throughput devices for optimizing protein drug formulations.