Immunostaining for allatotropin and allatostatin-A and -C in the mosquitoes Aedes aegypti and Anopheles albimanus

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• 作者：Salvador Hern¨¢ndez-Mart¨ªnez ; Yiping Li ; Humberto Lanz-Mendoza ; Mario H. Rodr¨ªguez and Fernando G. Noriega
• 关键词：Immunocytochemistry ; Neuropeptides ; Confocal laser ; scanning microscopy ; Mosquitoes Aedes aegypti ; Anopheles albimanus (Insecta)
• 刊名：Cell and Tissue Research
• 出版年：2005
• 出版时间：July 2005
• 年：2005
• 卷：321
• 期：1
• 页码：105-113
• 全文大小：551 KB

文摘

Confocal laser-scanning microscopy was used to carry out a comparative study of the immunostaining for three families of neuropeptides, viz., allatostatin-A (AS-A), allatostatin-C (AS-C) and allatotropin (AT), in adult female mosquitoes of Aedes aegypti and Anopheles albimanus. The specific patterns of immunostaining for each of the three peptides were similar in both species. The antisera raised against AT, AS-A, and AS-C revealed intense immunoreactivity in the cells of each protocerebral lobe of the brain and stained cells in each of the ventral ganglia and neuronal projections innervating various thoracic and abdominal tissues. Only the AS-A antiserum labeled immunoreactive endocrine cells in the midgut. The distribution of the peptides supports the concept that they play multiple regulatory roles in both species.