Microinjection of specific anti-IMPDH2 antibodies induces disassembly of cytoplasmic rods/rings that are primarily stationary and stable structures

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• 作者：Gerson Dierley Keppeke ; Luís Eduardo C Andrade ; Scott S Grieshaber…
• 关键词：CTP synthase ; Cytoophidium ; Inosine monophosphate dehydrogenase ; Intracellular compartment ; Mycophenolic acid ; Ribavirin
• 刊名：Cell & Bioscience
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：5
• 期：1
• 全文大小：1,754 KB
• 参考文献：1. Carcamo WC, Calise SJ, von Muhlen CA, Satoh M, Chan EK: Molecular cell biology and immunobiology of mammalian rod/ring structures. / Int Rev Cell Mol Biol 2014, 308:35?. CrossRef
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• 刊物主题：Cell Biology; Microbiology;
• 出版者：BioMed Central
• ISSN：2045-3701

文摘

Background Our laboratory previously reported interesting rods 3‰μm long and rings 2μm diameter (RR) in the cytoplasm of mammalian cells. Experimental evidence show that both inosine-5'-monophosphate dehydrogenase 2 (IMPDH2) and cytidine triphosphate synthetase (CTPS) are components of RR structures. Several cell types, including mouse embryonic stem cells, and cell lines, such as mouse 3T3 and rat NRK, naturally present RR structures, while other cells can present RR when treated with compounds interfering with GTP/CTP biosynthetic pathways. In this study, we aimed to investigate the dynamic behavior of these RR in live cells. Results RR were detected in >90% of COS-7 and HeLa cells treated with 1mM ribavirin or 6-Diazo-5-oxo-L-norleucine (DON) for 24h, and in 75% of COS-7 cells treated with 1mM mycophenolic acid (MPA) for the same period of time. Microinjection of affinity-purified anti-IMPDH2 antibodies in live COS-7 cells treated with ribavirin, DON, or MPA showed mature forms of RR presented as stable and stationary structures in 71% of cells. In the remaining 29% of cells, RR acquired erratic movement and progressively disassembled into fragments and disappeared within 10min. The specific stationary state and antibody-dependent disassembling of RR structures was independently confirmed in COS-7 and HeLa cells transfected with GFP-tagged IMPDH2. Conclusions This is the first demonstration of disassembly of RR structures upon microinjection of anti-IMPDH2 antibodies that led to the disappearance of the molecular aggregates. The disassembly of RR after microinjection of anti-IMPDH2 antibody further strengthens the notion that IMPDH2 are major building blocks of RR. Using two independent methods, this study demonstrated that the induced RR are primarily stationary structures in live cells and that IMPDH2 is a key component of RR.