Expression of immediate-early genes in the dorsal cochlear nucleus in salicylate-induced tinnitus

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• 作者：Shou-Sen Hu ; Ling Mei ; Jian-Yong Chen…
• 关键词：Tinnitus ; Arg3.1 ; Egr ; 1 ; NR2B ; Dorsal cochlear nucleus
• 刊名：European Archives of Oto-Rhino-Laryngology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：273
• 期：2
• 页码：325-332
• 全文大小：3,315 KB
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• 作者单位：Shou-Sen Hu (1) (2)
  Ling Mei (1) (2)
  Jian-Yong Chen (1) (2)
  Zhi-Wu Huang (1) (2)
  Hao Wu (1) (2)
  
  1. Department of Otolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai, 200092, China
  2. Ear Institute, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai, 200092, China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Otorhinolaryngology
  Neurosurgery
  Head and Neck Surgery
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1434-4726

文摘

Spontaneous neuronal activity in dorsal cochlear nucleus (DCN) may be involved in the physiological processes underlying salicylate-induced tinnitus. As a neuronal activity marker, immediate-early gene (IEG) expression, especially activity-dependent cytoskeletal protein (Arc/Arg3.1) and the early growth response gene-1 (Egr-1), appears to be highly correlated with sensory-evoked neuronal activity. However, their relationships with tinnitus induced by salicylate have rarely been reported in the DCN. In this study, we assessed the effect of acute and chronic salicylate treatment on the expression of N-methyl D-aspartate receptor subunit 2B (NR2B), Arg3.1, and Egr-1. We also observed ultrastructural alterations in the DCN synapses in an animal model of tinnitus. Levels of mRNA and protein expression of NR2B and Arg3.1 were increased in rats that were chronically administered salicylate (200 mg/kg, twice daily for 3, 7, or 14 days). These levels returned to baseline 14 days after cessation of treatment. However, no significant changes were observed in Egr-1 gene expression in any groups. Furthermore, rats subjected to long-term salicylate administration showed more presynaptic vesicles, thicker and longer postsynaptic densities, and increased synaptic interface curvature. Alterations of Arg3.1 and NR2B may be responsible for the changes in the synaptic ultrastructure. These changes confirm that salicylate can cause neural plasticity changes at the DCN level. Keywords Tinnitus Arg3.1 Egr-1 NR2B Dorsal cochlear nucleus